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Exploration of miRNAs and target genes of cytoplasmic male sterility line in cotton during flower bud development

文献类型： 外文期刊

作者： Nie, Hushuai 1 ; Wang, Yumei 2 ; Su, Ying 1 ; Hua, Jinping 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Lab Cotton Genet Genom & Breeding,Key Lab Crop He, Beijing Key Lab Crop Genet Improvement,Minist Edu, Beijing 100193, Peoples R China

2.Hubei Acad Agr Sci, Res Inst Cash Crops, Wuhan 430064, Hubei, Peoples R China

关键词： miRNA; Target gene; qRT-PCR; Flower bud; Cytoplasmic male sterility (CMS); Upland cotton

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN： 1438-793X

年卷期： 2018 年 18 卷 4 期

页码：

收录情况： SCI

摘要： Cytoplasmic male sterility (CMS) lines provide crucial material to harness heterosis for crop plants, which serves as an important strategy for hybrid seed production. However, the molecular mechanism remains obscure. Although microRNAs (miRNAs) play important roles in vegetative growth and reproductive growth, there are few reports on miRNAs regulating the development of male sterility in Upland cotton. In present study, 12 small RNA libraries were constructed and sequenced for two development stages of flower buds from a CMS line and its maintainer line. Based on the results, 256 novel miRNAs were allocated to 141 new miRNA families, and 77 known miRNAs belonging to 54 conserved miRNA families were identified as well. Comparative analysis revealed that 61 novel and 10 conserved miRNAs were differentially expressed. Further transcriptome analysis identified 232 target genes for these miRNAs, which participated in cellular developmental process, cell death, pollen germination, and sexual reproduction. In addition, expression patterns of typical miRNA and the negatively regulated target genes, such as PPR, ARF, AP2, and AFB, were verified by qRT-PCR in cotton flower buds. These targets were previously reported to be related to reproduction development and male sterility, suggesting that miRNAs might act as regulators of CMS occurrence. Some miRNAs displayed specific expression profiles in special developmental stages of CMS line and its fertile hybrid (F (1)). Present study offers new information on miRNAs and their related target genes in exploiting CMS mechanism, and revealing the miRNA regulatory networks in Upland cotton.

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