文献类型： 外文期刊

作者： Chen, Hong-Hao ¹ ; Dewer, Youssef ⁶ ; Wang, Yan ⁵ ; Tan, Shu-Qian ¹ ; Liu, Xiao-Li ⁵ ; Shi, Wang-Peng ¹ ;

作者机构： 1.China Agr Univ, Dept Entomol, Beijing, Peoples R China

2.China Agr Univ, MOA Key Lab Pest Monitoring, Beijing, Peoples R China

3.China Agr Univ, Green Management Chinese Med Herbs Res Ctr, Beijing, Peoples R China

4.China Agr Univ, Coll Plant Protect, Beijing, Peoples R China

5.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan, Peoples R China

6.Agr Res Ctr, Phytotox Res Dept, Cent Agr Pesticide Lab, Dokki, Egypt

关键词： beetle; antenna transcriptome; odorant receptor; RNA interference; licorice

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.755； 五年影响因子：5.316 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Introduction: Diorhabda tarsalis Weise is an important insect pest of the Chinese licorice Glycyrrhiza uralensis Fisch. Behavior of the beetle, including host location, oviposition site selection, self-defense, and aggregation, were regulated by plant volatiles or insect pheromones. Aim: In this study, Identification of ORs and function research on orco were carried out, these could lead to the development of understand for olfaction mechanism in D. tarsalis. Methods: ORs were identified by PacBio RS II platform to sequence the antennas of adult D. tarsalis, the function of orco was explored by dsRNA interference. Results: 29 odorant receptor candidate genes of D. tarsalis were obtained, which code for 130-479 amino acids. Phylogenetic trees of olfactory receptors were constructed with 243 ORs from eight Coleoptera species. DtarORco, DtarOR7 and DtarOR26 are specifically expressed in the antenna, and the expression levels were significantly higher than other DtarORs in antenna, there were no differential expression between male and female beetles. An odorant coreceptor gene (DtarORco) has characteristics of an odorant receptor family member, the encoded mature protein has a predicted molecular weight of 53.898 kDa, dsRNA L4440 expression vectors were constructed and successfully transformed into ribonuclease III-deficient Escherichia coli strain HT115 DE3. After interference treatment, the relative expression level of DtarORco in D. tarsalis antennae significantly decreased and electrophysiological responses to host localization odor signals significantly decreased. At the same time, beetles lost the ability to locate hosts. Discussion: The research on its mechanism of olfaction may lead to the development of new control measures that are environmentally friendly.