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Systematic Analysis of Long Non-Coding RNAs and mRNAs in the Ovaries of Duroc Pigs During Different Follicular Stages Using RNA Sequencing

文献类型： 外文期刊

作者： Liu, Yi 1 ; Li, Mengxun 1 ; Bo, Xinwen 2 ; Li, Tao 1 ; Ma, Lipeng 1 ; Zhai, Tenjiao 1 ; Huang, Tao 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, 221 North Fourth Rd, Shihezi 832000, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet, State Key Lab Sheep Genet Improvement & Hlth Prod, 221 Wu Yi Rd, Shihezi 832000, Peoples R China

关键词： RNA-seq; pig; ovary; lncRNA; mRNA

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 6 期

页码：

收录情况： SCI

摘要： The dynamic process involving the selection and maturation of follicles is regulated and controlled by a highly synchronized and exquisitely timed cascade of gene expression. Studies have shown that long non-coding RNA (lncRNA) is essential for the normal maintenance of animal reproductive function and has an important regulatory function in ovarian development and hormone secretion. In this study, a total of 2076 lncRNAs (1362 known lncRNAs and 714 new lncRNAs) and 25,491 mRNAs were identified in libraries constructed from Duroc ovaries on days 0, 2 and 4 of follicle development. lncRNAs were shorter, had fewer exons, exhibited a shorter ORF (Open Reading Frame) length and lower expression levels, and were less conserved than mRNAs. Furthermore, 1694 transcripts (140 lncRNAs and 1554 mRNAs) were found to be differentially expressed in pairwise comparisons. A total of 6945 co-localized mRNAs were detected in cis in 2076 lncRNAs. The most enriched GO (Gene Ontology) terms were related to developmental processes. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis revealed that the differentially expressed lncRNAs targeted mRNAs, and the differentially expressed mRNAs were related to the TGF- signaling pathway, the PI3K-Akt signaling pathway, the Retinol metabolic pathway and the Wnt signaling pathway. This study deepened our understanding of the genetic basis and molecular mechanisms of follicular development in pigs.

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