Cultivation of Drought-Tolerant and Insect-Resistant Rice Affects Soil Bacterial, but Not Fungal, Abundances and Community Structures
文献类型: 外文期刊
作者: Li, Peng 1 ; Ye, Shuifeng 3 ; Liu, Hua 1 ; Pen, Aihu 1 ; Ming, Feng 2 ; Tang, Xueming 1 ;
作者机构: 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China
2.Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn,Inst Plant Biol, Shanghai, Peoples R China
3.Shanghai Agrobiol Gene Ctr, Shanghai, Peoples R China
关键词: drought-tolerant and insect-resistant rice; Illumina MiSeq sequencing; Bt proteins; bacterial community composition; fungal community composition
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.64; 五年影响因子:6.32 )
ISSN: 1664-302X
年卷期: 2018 年 9 卷
页码:
收录情况: SCI
摘要: The impacts of rice varieties with stacked drought tolerance and insect resistance on soil microbiomes are poorly understood. Hence, the objective of this study was to investigate the effects resulting from the cultivation of the drought-tolerant and insect-resistant rice cultivar, Hanhui3T, on soil physical-chemical properties, and bacterial and fungal community composition. Soil samples of Hanhui3T and conventional rice varieties (Hanhui3 and Zhonghua11) were collected in triplicate at the booting stage, and bacterial and fungal population sizes and community structures were assessed using qPCR and Illumina MiSeq sequencing, respectively. The Bt protein concentration of Hanhui3T was significantly higher than that of Hanhui3 and Zhonghua11, while the pH of Hanhui3T was significantly lower. Bacterial population sizes and community composition were significantly different between Hanhui3T and Hanhui3 (or Zhonghua11), while no similar effects were observed for fungal communities. These differences suggest that the effect of Hanhui3T cultivation on bacterial community composition is stronger than the effect on fungal communities. Moreover, bacterial abundance was positively correlated to soil pH, while bacterial community structure variations were mainly driven by soil pH and Bt protein concentration differences. In conclusion, the abundances and structure of bacterial communities were altered in rhizosphere with Hanhui3T cultivation that changed soil pH and Bt protein concentrations, while fungal communities displayed no such responsiveness.
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