Evolution, expression, and characterisation of liver-expressed antimicrobial peptide genes in ancient chondrostean sturgeons
文献类型： 外文期刊
作者： Zhang, Shuhuan 1 ; Xu, Qiaoqing 2 ; Du, Hao 1 ; Qi, Zhitao 2 ; Li, Youshen 2 ; Huang, Jun 1 ; Di, Jun 1 ; Wei, Qiwei 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr China, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China
2.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Peoples R China
3.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Peoples R Chi
关键词： Antimicrobial peptide; Liver-expressed antimicrobial peptide 2; Innate immunity; Sturgeon
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2018 年 79 卷
页码：
收录情况： SCI
摘要： Liver-expressed antimicrobial peptide 2 (leap-2) is an evolutionarily ancient molecule that acts as the key component in vertebrate innate immunity against invading pathogens. Leap-2 has been identified and characterised in several teleosts, but not yet in chondrosteans. Herein, the complete coding sequences of leap-2b and leap-2c were identified from expressed sequence tags (ESTs) isolated from Dabry's sturgeon (Acipenser dabryanus) and Chinese sturgeon (A. sinensis), designated as adleap-2b, adleap-2c, asleap-2b, and asleap-2c, respectively. Adleap-2b and adleap-2c sequences share 98% and 100% sequence identity with asleap-2b, and asleap-2c, respectively. Sequence alignment revealed that all four genes contain four cysteine residues, conserved in all fish leap-2 homologs, that form two disulfide bonds. Comparative analysis of the exon-intron structure revealed a three exon/two intron structure for that leap-2 genes in animals, but intron 1 is much longer in sturgeons than in other species. The adleap-2c gene was expressed mainly in the liver of Dabry's sturgeon, and transcription of adleap-2c was significantly up-regulated (p < 0.05) in the liver and midkidney in response to Aeromonas hydrophila challenge. These results suggest adleap-2c may contribute to the defence against pathogenic bacterial invasion. The findings further our understanding of the function of adleap-2c and the molecular mechanism of innate immunity in chondrosteans.
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