文献类型： 外文期刊

作者： Wu, Wei-Jie ¹ ; Ahn, Byung-Yong ³ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Minist Agr, Key Lab Postharvest Handling Fruits, Hangzhou 310058, Zhejiang, Peoples R China

3.Chonbuk Natl Univ, Dept Oriental Med Resources, Iksan 570752, South Korea

关键词： Menaquinone-7; vitamin K-2; Bacillus subtilis; statistical optimization

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2018 年 28 卷 6 期

页码：

收录情况： SCI

摘要： Optimization of the culture medium to maximize menaquinone-7 (MK-7) production by Bacillus subtilis strain KCTC 12392BP in static culture was carried out using statistical experimental methods, including one factor at a time, fractional factorial design, and response surface methodology (RSM). Maltose (carbon source), tryptone (nitrogen source), and glycerol (activator) were identified as the key medium components for MK-7 synthesis by the fractional factorial design, and were selected for statistical optimization by RSM. The statistical analysis indicated that, in the range that was studied, maltose, tryptone, and glycerol were all critical factors having profound effects on the production of MK-7, with their coefficients for linear and quadratic all significant at the p < 0.05 level. The established model was efficient and feasible, with a determination coefficient (R-2) of 0.9419. The predicted concentrations of maltose, tryptone, and glycerol in the optimal medium were determined as 36.78, 62.76, and 58.90 g/l, respectively. In this optimized medium, the maximum yield of MK-7 reached a remarkably high level of 71.95 +/- 1.00 mu g/ml after 9 days of static fermentation, which further verified the practicability of this optimized strategy.