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Detection of viruses in abalone tissue using metagenomics technology

文献类型： 外文期刊

作者： Wei, Hong-Ying 1 ; Huang, Sheng 1 ; Yao, Tuo 1 ; Gao, Fang 1 ; Jiang, Jing-Zhe 1 ; Wang, Jiang-Yong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr, Key Lab Aquat Product Proc, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Shanghai, Peoples R China

3.Shanghai Ocean Univ, Shanghai, Peoples R

关键词： AbHV; AbSV; Haliotis diversicolor; high-throughput sequencing; viral metagenome; WSSV

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2018 年 49 卷 8 期

页码：

收录情况： SCI

摘要： Abalone (Haliotis diversicolor) is an important marine and mariculture food resource that has been severely affected by several viral disease outbreaks in recent years, and the virus causing the disease has not been unequivocally identified. To establish viral metagenomic research protocols for H.diversicolor, we applied differential centrifugation and sucrose gradient ultracentrifugation to obtain virus-like particles from crude tissue extracts. After nuclease treatment to remove host nucleic acid, reverse transcription, and double-stranded amplification, we used the HiSeq 2500 platform to generate a viral genome dataset. A total of 6,055,591,750 nucleotides were produced from 24,222,367 reads, generating 65,382 contigs, with a total sequence length of 14,239,389bp. We analysed and evaluated the sequence data by quality control mapping, among other methods and the results indicated good quality data. Alignment of the data with sequences in the GenBank virus database identified homology with a large number of Abalone Herpes-like Virus (AbHV) and Shriveling Syndrome-associated Virus (AbSV) sequences, and also a certain amount of White Spot Syndrome Virus (WSSV) sequences.

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