Two agroinfection-compatible fluorescent protein-tagged infectious cDNA clones of papaya leaf distortion mosaic virus facilitate the tracking of virus infection
文献类型： 外文期刊
作者： Tuo, D. C. 1 ; Yan, P. 1 ; Zhao, G. Y. 1 ; Li, X. Y. 1 ; Zhou, P. 1 ; Shen, W. T. 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou, Hainan, Peoples R China; Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China
关键词： PLDMV; fluorescent protein-tagged; agroinfectious clones
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2018 年 62 卷 2 期
页码：
收录情况： SCI
摘要： Papaya leaf distortion mosaic virus (PLDMV, the genus Potyvirus) is an emerging threat to papaya production. Here, agroinfection-compatible fluorescent protein-tagged PLDMV infectious cDNA clones driven by the Cauliflower mosaic virus 35S promoter were successfully constructed using one-step Gibson assembly. The clones were directly transformed into Agrobacterium tumefaciens to prevent potential problems such as plasmid instability during propagation in Escherichia coli. Ninety-five percent of papaya seedlings infected with PLDMV-GFP or PLDMV-mCherry developed systemic symptoms typical of those caused by wild-type PLDMV. Green and mCherry red fluorescence was observed in leaves, stems, and roots of infected papaya plants. The fluorescent protein-tagged agroinfectious PLDMV cDNA clones were stable in papaya for more than 90 days and during six serial passages at 30-day intervals. The availability of these infectious clones will contribute to research on PLDMV-host interactions and can be applied in the papaya breeding program for PLDMV resistance.
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