您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR

文献类型： 外文期刊

作者： Huo, P. 1 ; Shen, W. T. 1 ; Yan, P. 1 ; Tuo, D. C. 1 ; Li, X. Y. 1 ; Zhou, P. 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

关键词： papaya ringspot virus;papaya leaf distortion mosaic virus;papaya mosaic virus;multiplex real-time reverse transcription PCR

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Both the single infection of papaya ringspot virus (PRSV), papaya leaf distortion mosaic virus (PLDMV) or papaya mosaic virus (PapMV) and double infection of PRSV and PLDMV or PapMV which cause indistinguishable symptoms, threaten the papaya industry in Hainan Island, China. In this study, a multiplex real-time reverse transcription PCR (RT-PCR) was developed to detect simultaneously the three viruses based on their distinctive melting temperatures (Tms): 81.0 +/- 0.8 degrees C for PRSV, 84.7 +/- 0.6 degrees C for PLDMV, and 88.7 +/- 0.4 degrees C for PapMV. The multiplex real-time RT-PCR method was specific and sensitive in detecting the three viruses, with a detection limit of 1.0 x 10(1), 1.0 x 10(2), and 1.0 x 10(2) copies for PRSV, PLDMV, and PapMV, respectively. Indeed, the reaction was 100 times more sensitive than the multiplex RT-PCR for PRSV, and 10 times more sensitive than multiplex RT-PCR for PLDMV. Field application of the multiplex real-time RT-PCR demonstrated that some non-symptomatic samples were positive for PLDMV by multiplex real-time RT-PCR but negative by multiplex RT-PCR, whereas some samples were positive for both PRSV and PLDMV by multiplex real-time RT-PCR assay but only positive for PLDMV by multiplex RT-PCR. Therefore, this multiplex real-time RT-PCR assay provides a more rapid, sensitive and reliable method for simultaneous detection of PRSV, PLDMV, PapMV and their mixed infections in papaya.

  • 相关文献

[1]Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China. Tuo, D.,Li, Ch,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Li, H.. 2013

[2]Detection of Papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification. Shen, Wentao,Tuo, Decai,Yan, Pu,Li, Xiaoying,Zhou, Peng,Shen, Wentao,Tuo, Decai,Yan, Pu,Li, Xiaoying,Zhou, Peng,Tuo, Decai. 2014

[3]A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system. Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.. 2012

[4]Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system. Shen, W. T.,Wang, M. Q.,Yan, P.,Gao, L.,Zhou, P.. 2010

[5]Complete genome of Hainan papaya ringspot virus using small RNA deep sequencing. Zhang, Yuliang,Yu, Naitong,Huang, Qixing,Guo, Anping,Liu, Zhixin,Yin, Guohua,Wang, Xiangfeng,Xiong, Zhongguo,Wang, Xiangfeng,Xiong, Zhongguo.

[6]Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria. Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Yang, G..

[7]Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus. Zhou, Peng.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台