iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in Litopenaeus vannamei in response to infection with WSSV strains varying in virulence
文献类型： 外文期刊
作者： Sun, X. -Y. 1 ; Liu, Q. -H. 1 ; Huang, J. 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao Natl Lab Marine Sci & Technol,Qingdao Key, Minist Agr,Yellow Sea Fisheries Res Inst,Key Lab, Qingdao, Peoples R China
关键词： differential expression; iTRAQ; Litopenaeus vannamei; virulence; WSSV
期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）
ISSN： 0266-8254
年卷期： 2018 年 67 卷 2 期
页码：
收录情况： SCI
摘要： The isobaric tags for relative and absolute quantitation (iTRAQ) were applied to identify differentially expressed proteins (DEPs) in Litopenaeus vannamei in response to different virulence white spot syndrome virus infection. A total of 2780 unique peptides corresponding to 754 proteins were identified. The number of significant differentially expressed proteins was 161, including 38 up-regulated ones and 123 down-regulated ones in low-virulence infection library compared with normal-virulence infection library. Gene Ontology function annotation indicated that the differentially expressed proteins mainly participated in the biological process. Subcellular location classification showed that the largest distribution of differentially expressed proteins was found in the cytoplasm in both down-regulated and up-regulated proteins. Kyoto encyclopaedia of genes and genomes analysis revealed that most of the differentially expressed proteins were involved in carbon metabolism. Moreover, three metabolic pathways, including carbon metabolism, inositol phosphate metabolism, and fructose and mannose metabolism, were significantly affected. Our findings offered a better understanding of the host response to different virulence white spot syndrome virus infection. Significance and Impact of the Study: White spot syndrome virus (WSSV) is one of the most harmful pathogens in shrimp farming. Previous studies have shown that genetic variations of white spot syndrome virus cause the strains of different virulence. The hosts also show different self-regulation when infected by different viruses. A better understanding of host response to white spot syndrome virus will help elucidate the virulence and pathogenesis of this unique pathogen.
- 相关文献
作者其他论文 更多>>
-
Inactivated Mycobacterium vaccine induces innate immunity against M. ulcerans ecovar Liflandii (MuLiflandii ASM001) infection in Dabry's sturgeon (Acipenser dabryanus)
作者：Huang, J.;Wei, Q. W.;Huang, J.;Zhang, S. H.;Di, J.;Du, H.;Wei, Q. W.;Huang, J.
关键词：
-
Length-weight relationships of three fish species from the Tibet reach of the Lancang River, China
作者：Zhu, T. B.;Wang, X. G.;Huang, J.;Yang, D. G.
关键词：
-
Competition of infectious hypodermal and haematopoietic necrosis virus (IHHNV) with white spot syndrome virus (WSSV) for binding to shrimp cellular membrane
作者：Yan, D. C.;Liu, X.;Yan, D. C.;Huang, J.;Yang, B.;Sun, H. S.;Wang, Y. Y.
关键词：binding; cellular membrane; infectious hypodermal and haematopoietic necrosis (IHHNV); Litopenaeus vannamei; white spot syndrome virus (WSSV)
-
A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus
作者：Yang, H. -L.;Qiu, L.;Liu, Q.;Wan, X. -Y.;Liu, S.;Zhu, L. -L.;Yang, B.;Zhang, Q. -L.;Huang, J.;Yang, H. -L.;Qiu, L.;Liu, Q.;Liu, S.;Zhu, L. -L.;Yang, B.;Zhang, Q. -L.;Huang, J.
关键词：loop-mediated isothermal amplification; new genotype; polymerase chain reaction; shrimp; yellow head virus
-
Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp (Penaeus vannamei)
作者：Yang, Q.;Zou, P.;Xie, G.;Huang, J.;Yang, Q.;Fu, S.;Hao, J.;Fu, S.;Hao, J.;Wei, D.
关键词：AHPND; differentially expressed genes; insertion sequence; Vibrio parahaemolyticus; virulence factor
-
The establishment and application of isothermal cross-priming amplification techniques in detecting penaeid shrimp white spot syndrome virus
作者：Yang, H. -L.;Huang, J.;Yang, B.;Liu, F.;Zhang, Q. -L.;Yang, H. -L.;Huang, J.;Yang, B.;Zhang, Q. -L.;Yang, H. -L.;Huang, J.;Yang, B.;Zhang, Q. -L.
关键词：Cross-priming amplification;detect;polymerase chain reaction;white spot syndrome virus
-
VP37 of white spot syndrome virus interact with shrimp cells
作者：Liu, Q. -H.;Zhang, X. -L.;Ma, C. -Y.;Liang, Y.;Huang, J.
关键词：
