文献类型： 外文期刊
作者： Zuo, Jinhua 1 ; Wang, Yunxiang 3 ; Zhu, Benzhong 4 ; Luo, Yunbo 5 ; Wang, Qing 1 ; Gao, Lipu 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Minist Agr,Key Lab Vegetable Postharvest Treatmen, Beijing Key Lab Fruits & Vegetable Storage & Proc, Key Lab Urban Agr North,Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Boyce Thompson Inst Plant Res, Cornell Univ Campus, Ithaca, NY 14853 USA
3.Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China
4.China Agr Univ, Coll Food Sci & Nutr Engn, Lab Postharvest Mol Biol Fruits & Vegetables, Dept Food Biotechnol, Beijing 100083, Peoples R China
5.China Agr Univ, Coll Food Sci & Nutr Engn, Lab P
关键词： sRNAome; Transcriptome; Chilling response; Cowpea pods
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
ISSN： 0378-1119
年卷期： 2018 年 671 卷
页码：
收录情况： SCI
摘要： Cowpea is an important horticultural crop in tropical and subtropical areas of Asia, Africa, and Latin America, as well as parts of southern Europe and Central and South America. Chilling injury is a common physiological hazard of cowpea in cold chain logistics which reduce the cowpea pods nutritional quality and product value. However, the molecular mechanism involved in chilling injury remains unclear in cowpea pods. RNA-Seq and sRNA-Seq technologies were employed to decipher the miRNAs and mRNAs expression profiles and their regulatory networks in cowpea pods involved in chilling stress. Differentially expressed miRNAs and mRNA profiles were obtained based on cluster analysis, miRNAs and target genes were found to show coherent relationships in the regulatory networks of chilling injury. Furthermore, we found that numerous miRNAs and nat-siRNAs' targets were predicted to be key enzymes involved in the redox reactions such as POD, CAT, AO and LOX, energy metabolism such as ATPase, FAD and NAD related enzymes and different transcription factors such as WRKY, bHLH, MYB, ERF and NAC which play important roles in chilling injury.
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