Efficient transformation and expression of Vitreoscilla haemoglobin in the biological control bacterium Collimonas pratensis ZL261
文献类型： 外文期刊
作者： Zhang, Hong 1 ; Zheng, Rong 3 ; Zhang, Taotao 1 ; Dong, Dan 1 ; Liu, Ting 1 ; Zhao, Juan 1 ; Tian, Zhaofeng 1 ; Liu, Weic 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China
2.Inner Mongolia Normal Univ, Coll Life Sci & Technol, Hohhot, Peoples R China
3.Inner Mongolia Normal Univ, Coll Life Sci & Technol, Hohhot, Peoples R Ch
关键词： Collimonas pratensis; CO difference spectra; cell growth; antagonistic activity; Vitreoscilla haemoglobin
期刊名称：BIOCONTROL SCIENCE AND TECHNOLOGY （ 影响因子：1.665； 五年影响因子：1.616 ）
ISSN： 0958-3157
年卷期： 2018 年 28 卷 8 期
页码：
收录情况： SCI
摘要： Collimonas species are soil bacteria characterised by their ability to attach to and utilise fungi as a food source (mycophagy), as well as their chitin-degrading capacity (via chitinase production). These attributes, alongside volatile compounds, are thought to contribute to their function as fungal antagonists, including economically important plant pathogens. Despite this, studies have found no relationship between antifungal activity and chitinase production, or volatile compounds in Collimonaspratensis isolate ZL261, and there have been no studies on genetic control and regulatory biosynthesis of antifungal substances in Collimonas species. In this study, we showed that low concentrations of dissolved oxygen were unfavourable for growth and antifungal activity. We successfully introduced the gene vgb encoding Vitreoscilla haemoglobin (VHb) into isolate ZL261. The heterologous expression of VHb not only enhanced cell growth, but also improved antifungal activity against the brown rot fungus Monilinia fructicola under oxygen-restricted conditions; 18.6% of untreated peach fruits were infected (average lesion diameter: 9.2mm), while only 10.8% of fruit treated with the transformed isolate, ZV261, were infected (average lesion diameter: 5.4mm). These results suggest that the antagonism have been due to the secreted secondary metabolites, which are sensitive to the oxygen-restricted conditions.
- 相关文献
作者其他论文 更多>>
-
Diversity of Colletotrichum species associated with anthracnose on Euonymus japonicus and their sensitivity to fungicides
作者：Liu, Yayong;Tan, Xiaoqian;Zhao, Juan;Niu, Yajie;Qin, Wentao;Liu, Yayong;Zhao, Juan;Niu, Yajie;Qin, Wentao;Tan, Xiaoqian;Yu, Zhihe;Hsiang, Tom
关键词：Euonymus japonicus; Colletotrichum; anthracnose; fungicides; pathogenicity
-
Diversity of Trichoderma species associated with green mold contaminating substrates of Lentinula edodes and their interaction
作者：Cao, Zi-Jian;Zhao, Juan;Liu, Yu;Wang, Shou-Xian;Qin, Wen-Tao;Cao, Zi-Jian;Zheng, Su-Yue
关键词：Hypocreaceae; Trichoderma; green mold; Lentinula edodes; phylogeny; morphology; taxonomy
-
Rhizosphere-associated soil microbiome variability in Verticillium wilt-affected Cotinus coggygria
作者：Zhao, Juan;Cheng, Yanli;Jiang, Nan;Qiao, Guanghang;Qin, Wentao;Zhao, Juan;Qiao, Guanghang;Qin, Wentao;Cheng, Yanli;Jiang, Nan
关键词：Cotinus coggygria; Verticillium wilt; soil microbiome; rhizosphere; plant health; Illumina sequencing
-
Antagonistic Activity of Streptomyces alfalfae 11F against Fusarium Wilt of Watermelon and Transcriptome Analysis Provides Insights into the Synthesis of Phenazine-1-Carboxamide
作者：Dong, Dan;Zhang, Taotao;Niu, Zhenfeng;Zhao, Wenyu;Yu, Jiajia;Wu, Huiling;Li, Maoying;Xue, Guoping;Bai, Hongmei;Jiang, Wei
关键词：biological control; Streptomyces alfalfae; differentially expressed genes; secondary metabolites; RNA-seq
-
Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription
作者：Li, Maofu;Yang, Yuan;Wang, Hua;Fan, Youwei;Sun, Pei;Zhang, Hong;Zhang, Xinzhu;Jin, Wanmei;Li, Maofu;Yang, Yuan;Wang, Hua;Fan, Youwei;Sun, Pei;Zhang, Hong;Zhang, Xinzhu;Jin, Wanmei;Li, Maofu;Wang, Hua;Fan, Youwei;Sun, Pei;Zhang, Hong;Zhang, Xinzhu;Jin, Wanmei;Zhang, Hui;Xue, Zhen;Zhang, Hui;Xue, Zhen;Yang, Yuan
关键词：Rosa1 transposable element-like; RcMYB114; transcription; petal color; rose; anthocyanin
-
The characterization of Streptomyces alfalfae strain 11F and its effect on seed germination and growth promotion in switchgrass
作者：Niu, Zhenfeng;Su, Daolahu;Ma, Surina;Hu, La;Zhang, Taotao;Dong, Dan;Zhang, Dianpeng;Lu, Caige;Wu, Huiling;Yue, Yuesen;Hou, Xincun;Fan, Xifeng
关键词：Beneficial symbiotic microbes; Plant growth-promoting bacteria; Bioenergy crop; Green fluorescent protein; Colonization
-
Symmetric subgenomes and balanced homoeolog expression stabilize the establishment of allopolyploidy in cyprinid fish
作者：Ren, Li;Gao, Xin;Cui, Jialin;Zhang, Chun;Luo, Mengxue;Qin, Qinbo;Luo, Kaikun;Tao, Min;Xiao, Jun;Wang, Jing;Zhang, Hong;Zhang, Xueyin;Zhou, Yi;Wang, Shi;Hu, Fangzhou;Zhao, Rurong;Zhou, Rong;Wang, Yude;Liu, Qinfeng;Yan, Xiaojing;Wu, Chang;Yang, Conghui;Tang, Chenchen;Duan, Wei;Liu, Shaojun;Dai, He;Wang, Jing;He, Shaofang;Zhao, Xin;Liu, Guiming;Wang, Guoliang;Huo, Linhe
关键词：Polyploid; Hybridization; Genomic recombination; Cis- and trans- regulation; Fish
