Enzymatic Characterization of a Rumen Microorganism-Derived Multifunctional Glycoside Hydrolase and Its GH26 Domain with Mannanase Activity
文献类型： 外文期刊
作者： Zhao, Xin 1 ; Guo, Fangfang 2 ; Ma, Yong 1 ; Wang, Zhihong 1 ; Wu, Hao 1 ; Zhang, Hong 1 ; Gao, Li 1 ; Wen, Tong 1 ;
作者机构： 1.Baotou Teachers Coll, Fac Ecol & Environm, Baotou 014030, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing 100097, Peoples R China
关键词：
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 22 期
页码：
收录情况： SCI
摘要： In this study, a novel multifunctional glycoside hydrolase (GH) with two distinct domains homologous to the GH family 5 (GH5) and family 26 (GH26) was isolated from the rumen microorganism Segatella bryantii. The heterologous expression product of this enzyme exhibited both endo-beta-1,4-glucanase and endo-beta-1,4-mannanase activities. Intriguingly, segmental expression studies indicated that the GH26 domain alone contributed to the beta-mannanase activity, and its specific activity reached 2060 U/mg under optimal conditions (30 degrees C, pH 5.5). Furthermore, site-directed mutagenesis confirmed that the glutamic acid residues at positions 165 and 276 were indispensable for the catalytic activity of the GH26 domain. Collectively, a novel multifunctional GH from a symbiotic microorganism of ruminants was identified. Preliminary enzymatic characterizations of its GH26 family domain, which has independent beta-mannanase activities, were determined.
- 相关文献
作者其他论文 更多>>
-
Field study on the dynamic characteristics of sewage collection in a circular aquaculture tank
作者：Wu, Yanfei;Zhang, Qingjing;Gui, Fukun;Feng, Dejun;Yang, Jianxin;Qi, Hongfang;Zhang, Hong;Wang, Yang
关键词：Circular aquaculture tank; Field study; Hydrodynamics; Inlet; Sewage collection
-
U2Net-MGP: A Lightweight and Efficient Visual Perception Algorithm for Consumer Electronic Accessories
作者：Chen, Wenbai;Zhang, Bo;Zhao, Xin;Wang, Yiqun;Li, Jingchen;Shi, Haobin;Gou, Jianping
关键词：Image segmentation; Consumer electronics; Feature extraction; Assembly; Accuracy; Computational modeling; Decoding; salient object segmentation; ghost convolution; polarized self-attention mechanism; multi-scale feature fusion
-
Zinc methionine mitigates intestinal mucosal injury of broilers challenged with Salmonella by modulating mucus secretion, tight junction and microbial composition
作者：Shao, Yuxin;Wang, Zheng;Sun, Huiling;Cui, Yifang;Guo, Fangfang;Xu, Fuzhou;Suo, Yanrui;Lin, Dongmei;Ma, Tenghe
关键词：Zinc methionine; Salmonella; Mucus; Intestinal microbiota; Zinc methionine; Salmonella; Mucus; Intestinal microbiota
-
Assessment of probiotic Bacillus velezensis supplementation to reduce Campylobacter jejuni colonization in chickens
作者：Cui, Yifang;Li, Pengxiang;Guo, Fangfang;Yang, Bing;Su, Xia;Zhou, Hongzhuan;Xu, Fuzhou;Zhu, Jiajia;Zhu, Kui
关键词：probiotic; Bacillus velezensis; antimicrobial activity; Campylobacter jejuni; chicken
-
Effects of OxyR regulator on oxidative stress, Apx toxin secretion and virulence of Actinobacillus pleuropneumoniae
作者：Guo, Fangfang;Quan, Rong;Cui, Yifang;Cao, Xiaoya;Xu, Fuzhou;Wen, Tong
关键词：Actinobacillus pleuropneumoniae; oxyR gene; Apx toxins; oxidative stress; virulence
-
Risk assessment of Bacillus cereus in lettuce grown in open-field and greenhouse farming systems in China: A comparative study
作者：Qu, Yang;Zhou, Changyan;Suo, Yujuan;Yu, Shenghao;Gu, Haotian;Wang, Wen;Wang, Wenbo;He, Zhaoying;Shan, Hong;Gao, Min;Zhao, Xin;Wei, Chao;Tao, Tingting;Zhao, Guanghua
关键词：Diarrheal syndrome food poisoning; ready-to-eat vegetable; greenhouse; microbial risk assessment
-
Sustainable on-farm strategy for the disposal of antibiotic fermentation residue: Co-benefits for resource recovery and resistance mitigation
作者：Zhou, Jieya;Hou, Lian;Zhou, Jieya;Wu, Hao;Shi, Lihu;Tian, Shulei;Wang, Xuming;Shen, Yunpeng;Hou, Lian
关键词：Antibiotic resistance; Bio-organic fertilizers; Antibiotic resistance genes; Mobile genetic elements
