Novel two-stage solid-state fermentation for erythritol production on okara-buckwheat husk medium
文献类型： 外文期刊
作者： Liu Xiaoyan 1 ; Yu Xinjun 3 ; Zhang Tong 1 ; Wang Zhipeng 4 ; Xu Jiaxing 1 ; Xia Jun 1 ; He Aiyong 1 ; Yan Yubo 1 ; Xu Jimin 1 ;
作者机构： 1.Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian, Jiangsu, Peoples R China
2.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Huaian, Jiangsu, Peoples R China
3.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Key Lab Bioorgan Synth Zhejiang Prov, Hangzhou, Zhejiang, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
关键词： Two-stage SSF; Erythritol fermentation; Okara-buckwheat husk medium; Mucor flavus; Yarrowia lipolytica
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2018 年 266 卷
页码：
收录情况： SCI
摘要： An economical model of two-stage solid state fermentation (SSF) (prefermentation stage with Mucor flavus and in situ erythritol fermentation stage with Yarrowia lipolytica) for enhancing erythritol production was investigated. Buckwheat husk (BH) was utilized as inert support for the first time and okara as the substrate. Morphological properties suggested yeast cells were exposed in adequate oxygen leading to high erythritol yield, and enzyme activities analysis indicated M. flavus and Y. lipolytica grew and cooperated well during the two ferment stages. Maximum erythritol production (143.3 mg/gds) was obtained from okara-BH mixture (5:2, w/w) supplemented with 0.01 g/gds NaCl, with an initial moisture content of 60% and pH of 4.0 for 192 h, while undesired mannitol and citric acid were suppressed. Compared with submerged fermentation, two-stage SSF was short period, energy conserving and operable for erythritol production from insoluble wastes, and this is the first report on erythritol production via SSF.
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