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Determination of gentamicin C components in fish tissues through SPE-Hypercarb-HPLC-MS/MS

文献类型： 外文期刊

作者： Sun, Xiaojie 1 ; Yang, Yuanhao 3 ; Tian, Qiangbing 3 ; Shang, Derong 1 ; Xing, Jun 5 ; Zhai, Yuxiu 1 ;

作者机构： 1.Minist Agr, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Chinese Acad Fishery Sci, Shaanxi Fisheries Inst, Xian 710086, Shaanxi, Peoples R China

4.Chinese Acad Fishery Sci, Yellow River Fisheries Res Inst, Xian 710086, Shaanxi, Peoples R China

5.Wuhan Univ, Coll Chem & Mol Sci, Key Lab Analyt Chem Biol & Med, Minist Educ, Wuhan 430072,

关键词： Gentamicin C components; Fish tissue; Porous graphite carbon column; Strong cation exchange; HPLC-MS/MS

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN： 1570-0232

年卷期： 2018 年 1093 卷

页码：

收录情况： SCI

摘要： In this work, an HPLC/MS/MS method for determination of gentamicin C components in fish tissues was developed based on strong cation exchange solid-phase extraction (SPE) purification coupled with Hypercarb chromatographic column in separation mode. Sample was extracted using trichloroacetic acid aqueous solution containing EDTA. Ion-pairing reagents were not needed because of the "graphite polarity retention effect" of the Hypercarb chromatographic column. HPLC-MS/MS was performed in multiple reaction monitoring (MRM) mode for simultaneous qualitative and quantitative analyses (using matrix external standard) of gentamicin C components in fish tissues. Good linearity was obtained for the target analytes within the concentration range from 0.0100 to 0.500 mg/L. The limits of quantification (LOQ) of this method were 10.0, 20.0, and 20.0 mu g/kg for C1, C1a, and sum of C2 + C2a, respectively. The average recoveries of gentamicin C components were 80.0%-110% when spiked at three levels with the blank carp (Cyprinus carpio) matrix, and the relative standard deviations (RSD) were all less than 15% (n = 6). In addition, for the features of simple operation, high sensitivity and good reproducibility, the proposed method has been successfully applied for detection of gentamicin residues in fish tissues during actual breeding.

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