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Inter- and Intra-Specific Differentiation of Trichogramma (Hymenoptera:Trichogrammatidae) Species Using PCR-RFLP Targeting COI

文献类型： 外文期刊

作者： Hua, Hai-Qing 1 ; Zhao, Zi-Yi 1 ; Zhang, Yang 1 ; Hu, Jin 1 ; Zhang, Fan 2 ; Li, Yuan-Xi 1 ;

作者机构： 1.Nanjing Agr Univ, Dept Entomol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Trichogramma(Hymenoptera: Trichogrammatidae); morphological character; mtCOI; PCR-RFLP; distinguish

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN： 0022-0493

年卷期： 2018 年 111 卷 4 期

页码：

收录情况： SCI

摘要： The identification of Trichogramma (Hymenoptera: Trichogrammatidae) species is problematic due to their small size and lack of distinct morphological characters. In this study, we combined morphological characters of the male genitalia and molecular methods using the mitochondrial cytochrome oxidase I (COI) gene as a molecular marker to identify eight species from 16 geographic populations: T. evanescens Westwood, T. cacoeciae Marchal, T. ostriniae Pang et Chen, T. chilonis Ishii, T. japonicum Ashmead, T. brassicae Bezdenko, T. bilingensis He et Pang, and T. dendrolimi Matsumura. We developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method targeting the mitochondrial COI to distinguish the eight Trichogramma species using three restriction enzymes. We further analyzed 109 COI fragments from 18 Trichogramma species and found that the PCR-RFLP method could distinguish both intra- and inter-specific genetic variation among most of the species using four additional restriction enzymes.

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