Purification and molecular characterization of a Metschnikowia saccharicola killer toxin lethal to a crab pathogenic yeast
文献类型： 外文期刊
作者： Tan, Chunming 1 ; Wang, Lin 3 ; Xue, Yong 2 ; Lin, Shuo 4 ; Yu, Gang 1 ; Yang, Shaoling 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Natl Res & Dev Ctr Aquat Prod Proc, Key Lab Aquat Prod Proc,Minist Agr, Guangzhou 510300, Guangdong, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
3.Beihang Qingdao Res Inst, Beihang Goertec Microelect Inst, Qingdao 266041, Peoples R China
4.Air Liquide Med Syst, Dept Qual & Regulatory Affairs, F-92182 Antony, France
关键词： killer toxin; purification; molecular characterization; Metschnikowia saccharicola; pathogenic yeast
期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）
ISSN： 0378-1097
年卷期： 2018 年 365 卷 10 期
页码：
收录情况： SCI
摘要： The marine yeast strain Metschnikowia saccharicola DD21-2, isolated from sediments in the Yalu River, produces a killer toxin with a lethal effect on Metschnikowia bicuspidate strain WCY, a pathogenic yeast strain that infects crabs. In this study, the killer toxin was purified and characterized. After sequential purification, the purity of the killer toxin was increased 72.2-fold over the purity of the yeast cell culture supernatant. The molecular weight of the purified killer toxin was 47.0 kDa. The optimal pH and temperature for killing activity were 5.5 degrees C and 16 degrees C, respectively. The killing activity was stable over a pH range of 4.0-6.5 and temperature range of 0 degrees C-40 degrees C. The purified killer toxin was only effective against toxin-sensitive integral cells and had no killing effect on the protoplasts of toxin-sensitive cells. When exerting the killing effect, the toxin bind to a cell wall receptor of the treated strain, disrupted cell wall integrity and eventually caused death. The amino acid sequence identified by mass spectroscopy indicated that the purified killer toxin might be a protein kinase, but did not show beta-1,3-glucanase activity, consistent with the laminarin hydrolysis results. These findings provide a basis for disease prevention and control in marine aquaculture.
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