文献类型： 外文期刊

作者： Zhao, Qian ¹ ; Shen, Weijun ¹ ; Chen, Quan ³ ; Helmisaari, Helja-Sisko ⁵ ; Sun, Qiaoqi ⁶ ; Jian, Shuguang ¹ ;

作者机构： 1.Chinese Acad Sci, South China Bot Garden, Guangdong Prov Key Lab Appl Bot, Guangzhou 510650, Guangdong, Peoples R China

2.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Key Lab Marine Ranch Technol, Guangzhou 510300, Guangdong, Peoples R China

4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

5.Univ Helsinki, Dept Forest Sci, Helsinki 00790, Finland

6.Univ Adelaide, Sch Biol Sci, Dept Ecol & Environm Sci, Adelaide, SA 5005, Australia

关键词： Precipitation change; Soil fungi; Soil bacteria; PLFAs; Microbial DNA sequencing; Microbial function

期刊名称：APPLIED SOIL ECOLOGY （ 影响因子：4.046； 五年影响因子：4.884 ）

ISSN： 0929-1393

年卷期： 2018 年 130 卷

页码：

收录情况： SCI

摘要： The predicted changes in the seasonal precipitation pattern could influence the soil microbial communities composition and function. However, the responses of soil microbes to seasonal changes in precipitation are poorly understood, especially in subtropical forests with distinct dry-wet seasons. A manipulation experiment lasted for two years to determine the effects of reduced rainfall in spring and increased rainfall in summer (the wet season) on the composition and function of soil microbial community in a subtropical forest. We excluded 67% of throughfall during spring and then added the equivalent amount of water back to the exclusion plots in intense rainfall events during summer. Throughfall exclusion reduced the soil water content (SWC) by 4.8-6.5%, and water addition increased the SWC by 12.5% but without statistical significance. However, wet-season water addition significantly decreased the ratio of fungal to bacterial phospholipid fatty acids by 12.7% in a relatively dry year (2014). Amplicon sequencing indicated that water addition increased the rare bacterial phylum Gemmatimonadetes, which accounted for 0.59-0.73% of the total OTUs in the soil. Water addition also increased Basidiomycota and decreased Ascomycota; these two phyla accounted for 71-86% of the total fungal OTUs in the soil. Soil enzyme activities were unaffected by reduced rainfall in spring and increased rainfall in summer, expect that beta-1,4-glucosidase activity which was positively related to SWC. Our results suggest that bacteria are more responsive than fungi to reduced rainfall in the spring, while fungi are more responsive than bacteria to increased rainfall in the summer. To clarify how microbial functions change in response to changes in precipitation, future research should assess the expression of functional genes.