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Isolation and Diversity of Actinomycetes from Sediments of Different Depths Between 34 m and 3,235 m in South China Sea

文献类型： 外文期刊

作者： Kamjam, Manita 1 ; Xie, Qingyi 3 ; Deng, Zixin 1 ; Hong, Kui 1 ;

作者机构： 1.Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Hubei, Peoples R China

2.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China

3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

关键词： marine actinomycetes; isolation; diversity; deep sea

期刊名称：CHIANG MAI JOURNAL OF SCIENCE （ 影响因子：0.523； 五年影响因子：0.505 ）

ISSN： 0125-2526

年卷期： 2018 年 45 卷 4 期

页码：

收录情况： SCI

摘要： Diversity and isolation of actinomycetes from marine sediments collected from the South China Sea at depths ranging from 34 to 3,235 m were carried using dilution plate technique with heat and non-heat pretreatment on 9 isolation media, and plate stamping technique incubated on RH and M6 media at 28 degrees C and 10 degrees C. A total of 43 actinomycete strains were isolated from the different depth of marine sediments, among of which 32 were from the deep sea samples (1,645-3,235 m). Non-heat pretreatment was effective for the actinomycete isolation from deep sea sediment samples. RH and M6 were the more effective media regarding the number and diversity of isolates recovered. A higher percentage of actinomycete colonies (19.64%) were obtained by using plate stamping technique comparing to dilution plate method (5.29%). However, low-temperature incubation (10 degrees C) was also significantly effective on actinomycetes isolation from deep-sea samples with a higher percentage of the ratio actinomycetes to other total viable bacteria. Higher diversity of actinomycetes genera were found in deep-sea regions (depth 1,645 m-3,235 m) comparing to shallow regions marine sediments (depth < 200 m). Partial 16S rRNA gene sequence data revealed that the isolates belong to the Class Actinobacteria, with genetic affiliations to five orders, six families, and six genera. Micromonospora (n= 23) and Streptomyces (n= 14) were the dominant genera, followed by Dietzia (n=2), Tsukamurella (n=2), Blastococcus (n=l) and Microbacterium (n=1).

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