文献类型： 外文期刊

作者： Chen, Yiping ¹ ; Cao, Jing ¹ ; Jiang, Xiaoxue ¹ ; Pan, Zhizhen ³ ; Fu, Nanyan ¹ ;

作者机构： 1.Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China

2.Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Fujian, Peoples R China

3.Fujian Acad Agr Sci, Agr Bioresources Res Inst, Fuzhou 350003, Fujian, Peoples R China

关键词： Chymotrypsin; 1,8-Naphthalimide; Intramolecular charge transfer (ICT); Ratiometric fluorescence probe; Screening chymotrypsin inhibitor

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN： 0925-4005

年卷期： 2018 年 273 卷

页码：

收录情况： SCI

摘要： Chymotrypsin (CHT) is involved in a series of physiological processes and disorders and it is known as a biomarker of pancreatic function. Thus, a sensitive and selective method for evaluating the relative CHT levels in different live cells is urgently needed to better understand the function of CHT. Herein, a ratiometric fluorescence probe (NI) with strong intramolecular charge transfer (ICT) has been rationally designed. The probe is constructed by incorporating a recognition group of 4-bromobutyryl into the fluorescent skeleton of 1,8-naphthalimide. The presence of CHT cleaves the 4-bromobutyryl group from naphthalimide and remarkably alters the probe's photophysical properties with a large Stokes shift, thus achieving ratiometric detection of CHT. With a detection limit of 8.4 ng/mL, NI shows high binding affinity and comparable catalytical efficiency against CHT and was successfully applied for the inhibitor characterization. The results also demonstrate that the designing strategy in the text provides a more promising application prospect for screening others CHT inhibitors.