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Mass spider silk production through targeted gene replacement in Bombyx mori

文献类型： 外文期刊

作者： Xu, Jun 1 ; Dong, Qinglin 2 ; Yu, Ye 1 ; Niu, Baolong 4 ; Ji, Dongfeng; Li, Muwang; Huang, Yongping 1 ; Chen, Xin 2 ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Ctr Excellence Mol Plant Sci, Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China

2.Fudan Univ, State Key Lab Mol Engn Polymers, Lab Adv Mat, Shanghai 200433, Peoples R China

3.Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China

4.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peopl

关键词： genome editing; TALEN; spider silk; Bombyx mori

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

ISSN： 0027-8424

年卷期： 2018 年 115 卷 35 期

页码：

收录情况： SCI

摘要： Spider silk is one of the best natural fibers and has superior mechanical properties. However, the large-scale harvesting of spider silk by rearing spiders is not feasible, due to their territorial and cannibalistic behaviors. The silkworm, Bombyx mori, has been the most well known silk producer for thousands of years and has been considered an ideal bioreactor for producing exogenous proteins, including spider silk. Previous attempts using transposon-mediated transgenic silkworms to produce spider silk could not achieve efficient yields, due to variable promoter activities and endogenous silk fibroin protein expression. Here, we report a massive spider silk production system in B. mori by using transcription activator-like effector nuclease-mediated homology-directed repair to replace the silkworm fibroin heavy chain gene (FibH) with the major ampullate spidroin-1 gene (MaSp1) in the spider Nephila clavipes. We successfully replaced the similar to 16-kb endogenous FibH gene with a 1.6-kb MaSp1 gene fused with a 1.1-kb partial FibH sequence and achieved up to 35.2% chimeric MaSp1 protein amounts in transformed cocoon shells. The presence of the MaSp1 peptide significantly changed the mechanical characteristics of the silk fiber, especially the extensibility. Our study provides a native promoter-driven, highly efficient system for expressing the heterologous spider silk gene instead of the transposon-based, random insertion of the spider gene into the silkworm genome. Targeted MaSp1 integration into silkworm silk glands provides a paradigm for the large-scale production of spider silk protein with genetically modified silkworms, and this approach will shed light on developing new biomaterials.

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