文献类型： 外文期刊

作者： Xu, Xiaojun ¹ ; Yu, Jiongying ² ; Ge, Jianhui ¹ ; Yi, Shaokui ³ ; Weng, Xudong ⁴ ; Guan, Wenzhi ¹ ; Niu, Baolong ¹ ; Zhang, Xinhui ⁵ ; Lou, Bao ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Inst Hydrobiol, Hangzhou 310021, Peoples R China

2.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Key Lab Freshwater Anim Breeding,Minist Educ,Minis, Wuhan 430070, Peoples R China

3.Huzhou Univ, Sch Life Sci, Huzhou 313000, Peoples R China

4.Zhejiang Fishery Boss Agr Technol Co Ltd, Quzhou 324000, Peoples R China

5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

6.BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ An, BGI Marine,BGI Acad Marine Sci, Shenzhen 518083, Peoples R China

关键词： Opsariichthys bidens; Sex; Growth; GWAS; Sex-specific marker

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2024 年 35 卷

页码：

收录情况： SCI

摘要： Chinese hooksnout carp (Opsariichthys bidens) is an endemic stream fish and has begun to be artificially bred and cultivated due to its superior taste, high economic value and fast growth. An obvious sex dimorphism in growth has been observed in O. bidens. To explore the genetic architecture underlying the sex and growth traits, genome-wide association studies (GWAS) for sex and growth traits with a O. bidens aquaculture population consisting of 99 males and 101 females were performed using whole-genome re-sequencing. For the sex trait, a total of 5744 SNP loci (P value < 1 x 10(-6)) were found to be significantly associated with sex, 92.41 % (5308) of which were clustered on chromosome 24 within a 15.37 Mb region, and six candidate sex-related genes were detected, including sox4, nr0b2, u2af2a, rspo1, spaca6, tdrp-like. Almost all SNPs significantly associated with sex were heterozygous in males and homozygous in females. Therefore, we speculated that Chinese hooksnout carp had an XX/XY sex determination system. For the growth trait, a total of 80 SNP loci (P value < 1 x 10(-6)) were found to be significantly associated with the growth trait, which were unevenly distributed on chromosome 17, 11, 4 and 8. Similarly, six candidate growth-related genes were detected, including chst12, neub-like,nckx2, eif3f, eif3b, fgb-like. To develop a sex-specific marker, we performed a genome comparison between male and female genome. A male-specific insert of 2648 bp located on chromosome 24 was detected, and finally a PCR-based sex identification method was constructed based on the male-specific insert. Overall, this study explored the genetic basis of the sex and growth traits in O. bidens, and the identification of a sex-specific marker provided a powerful tool for the breeding of a mono-sexual strain.