文献类型： 外文期刊

作者： Sun, Fei ¹ ; Tu, Rongjian ¹ ; Xia, Jun Hong ¹ ; Liu, Xiao Jun ¹ ; Yue, Gen Hua ¹ ;

作者机构： 1.Natl Univ Singapore, Temasek Life Sci Lab, 1 Res Link, Singapore 117604, Singapore

2.Shanghai Acad Agr Sci, Inst Crop Breeding & Cultivat, Shanghai 201403, Peoples R China

3.Sun Yat Sen Univ, Inst Aquat Econ Anim, Coll Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

4.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China

5.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

6.Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr, Singapore 117543, Singapore

7.Nanyang Technol Univ, Sch Biol Sci, 6 Nanyang Dr, Singapore 637551, Singapo

关键词： FTO; Polymorphism; Trait; Breeding; Aquaculture

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN： 1436-2228

年卷期： 2018 年 20 卷 5 期

页码：

收录情况： SCI

摘要： Polymorphisms in the FTO gene are associated with obesity and body mass index in humans and livestock. Little information of whether FTO plays an important role in aquaculture fish species is available. We cloned and characterized the FTO gene in an economically important food fish species: Asian seabass (Lates calcarifer). The full-length cDNA of the gene is 3679bp, containing an ORF of 1935bp encoding 644 amino acids, a 216bp 5 UTR and a 1538bp 3 UTR. The gene consisted of nine exons and eight introns and was 117,679bp in length. Phylogenetic analysis revealed that the gene in Asian seabass was closely related to those of Japanese flounder and Nile tilapia. Analysis of its expressions using qRT-PCR showed that it was expressed ubiquitously, but was higher in the liver, stomach and intestine. Comparative analysis of the genomic sequences of part of intron 1 of the gene among 10 unrelated individuals identified two SNPs. Analysis of associations between SNPs and traits (i.e. growth, oil content, omega-3 and -6 contents) in an F-2 family demonstrated that the two SNPs were significantly associated with growth, oil content, omega-3 content and omega-3/-6 ratio. Altogether, our data suggest that the gene or/and its linked genes play an important role in growth and fatty acid synthesis, and that the SNPs associated with traits may be used as markers for selecting quicker growth and higher omega-3/-6 ratio at the fingerling stage.