文献类型： 外文期刊

作者： Du, Gai Mei ¹ ; Luo, Bi Ping ¹ ; Hu, Zhi Hua ¹ ; Wu, Jie Ge ¹ ; Yan, Wen Mei ¹ ; Han, Zheng Qiang ¹ ; Zhang, Yu Hong ¹ ;

作者机构： 1.Jinling Technol Inst, Dept Anim Sci & Technol, Nanjing 210038, Jiangsu, Peoples R China

2.Minist Agr, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci,Natl Ctr Engn Res Vet Biopro, Nanjing 210014, Jiangsu, Peoples R China

3.State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing, Jiangsu, Peoples R China

关键词： GOAT; H+-K+-ATPase activity; Acylated ghrelin; Total ghrelin

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN： 0016-6480

年卷期： 2018 年 267 卷

页码：

收录情况： SCI

摘要： Ghrelin is implicated in the regulation of gastric functional development. The octanoylation of ghrelin is critical for its physiological functions which dependent upon ghrelin O-acyltransferase (GOAT) catalyzation. To investigate the effect of GOAT on gastric acid secretion and expression of ghrelin in vitro. Primary cultures of gastric mucosal cells were challenged with 1.5 x 10(-5), 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (The GOAT inhibitor), respectively, for 24 h in order to further clarify the effect of GOAT on H+-K+-ATPase activity. In vitro, GO-CoA-Tat significantly increased ghrelin and GOAT mRNA expression at 1.5 x 10(-5), 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL, and augmented cell total ghrelin secretion at 1.5 x 10(-3) mol/mL. But cell acylated ghrelin secretion was reduced at 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). And cell acylated ghrelin synthesis was reduced at 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). In accordance with acylated ghrelin level, H+-K+-ATPase activity were decreased with 1.5 x 10(-4) and 1.5 x 10(-3) mol/mL GO-CoA-Tat (P < 0.05). These results indicated that GOAT inhibitor decreases the acylated ghrelin level and H+-K+-ATPase activity in vitro.