Screening of potential IL-tolerant cellulases and their efficient saccharification of IL-pretreated lignocelluloses
文献类型： 外文期刊
作者： Sun, Yi-Xin 1 ; Shen, Bing-Bing 1 ; Han, Hui-Ying 1 ; Lu, Yuan 1 ; Zhang, Bi-Xian 2 ; Gao, Yun-Fei 2 ; Hu, Bao-Zhong 3 ;
作者机构： 1.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Harbin 150086, Heilongjiang, Peoples R China
3.Harbin Univ, Harbin 150086, Heilongjiang, Peoples R China
期刊名称：RSC ADVANCES （ 影响因子：3.361； 五年影响因子：3.39 ）
ISSN： 2046-2069
年卷期： 2018 年 8 卷 54 期
页码：
收录情况： SCI
摘要： Lignocellulosic biomass as one of the most abundant and renewable resources has great potential for biofuel production. The complete conversion of biomass to biofuel is achieved through the effective pretreatment process and the following enzyme saccharification. Ionic liquids (ILs) are considered as a green solvent for lignocellulose pretreatment. However, ILs exhibit an inhibitory effect on cellulase activity, leading to a subsequent decrease in the efficiency of saccharification. The screening of new potential IL-tolerant cellulases is important. In the current study, a fungal strain with a relatively high cellulase production was isolated and identified as Penicillium oxalicum HC6. The culture conditions were optimized using corn stover and peptone as the carbon source and nitrogen source at pH 4.0 and 30 degrees C with an inoculation size of 2% (v/v) for 8 days. It was found that P. oxalicum HC6 exhibited potential salt tolerance with the increase of the enzyme production at a salt concentration of 5.0% (w/v). In addition, high enzyme activities were obtained at pH 4.0-6.0 and 50-65 degrees C. The crude enzyme from P. oxalicum HC6 with good thermal stability was also stable in the presence of salt and ILs. Good yields of reducing sugar were obtained by the crude enzyme from P. oxalicum HC6 after the saccharification of corn stover that was pretreated by ILs. P. oxalicum HC6 with potentially salt-tolerant and IL-tolerant enzymes has great potential application in the enzymatic saccharification of lignocellulose.
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