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Molecular characterization and expression of six heat shock protein genes in relation to development and temperature in Trichogramma chilonis

文献类型： 外文期刊

作者： Yi, Jiequn 1 ; Wu, Han 1 ; Liu, Jianbai 1 ; Lai, Xueshuang 1 ; Guo, Jixing 1 ; Li, Dunsong 3 ; Zhang, Guren 1 ;

作者机构： 1.Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Inst Entomol, Guangzhou, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Technol Plant Protect, Plant Protect Res Inst, G

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 9 期

页码：

收录情况： SCI

摘要： Trichogramma is a kind of egg parasitoid wasp that is widely used to control lepidopterous pests. Temperature is one of the main factors that determines the various life activities of this species, including development, reproduction and parasitism efficiency. Heat shock proteins (HSPs) are highly conserved and ubiquitous proteins that are best known for their responsiveness to temperature and other stresses. To explore the potential role of HSPs in Trichogramma species, we obtained the full-length cDNAs of six HSP genes (Tchsp10, Tchsp21.6, Tchsp60, Tchsp70, Tchsc70-3, and Tchsp90) from T. chilonis and analyzed their expression patterns during development and exposure to temperature stress. The deduced amino acid sequences of these HSP genes contained the typical signatures of their corresponding protein family and showed high homology to their counterparts in other species. The expression levels of Tchsp10, Tchsp21.6 and Tchsp60 decreased during development. However, the expression of Tchsc70-3 increased from the pupal stage to the adult stage. Tchsp70 and Tchsp90 exhibited the highest expression levels in the adult stage. The expression of six Tchsps was dramatically upregulated after 1 h of exposure to 32 and 40 degrees C but did not significantly change after 1 h of exposure to 10 and 17 degrees C. This result indicated that heat stress, rather than cold stress, induced the expression of HSP genes. Furthermore, the expression of these genes was time dependent, and the expression of each gene reached its peak after 1 h of heat exposure (40 degrees C). Tchsp10 and Tchsp70 exhibited a low-intensity cold response after 4 and 8 h of exposure to 10 degrees C, respectively, but the other genes did not respond to cold at any time points. These results suggested that HSPs may play different roles in the development of this organism and in its response to temperature stress.

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