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Effects of mixing red clover with alfalfa at different ratios on dynamics of proteolysis and protease activities during ensiling

文献类型： 外文期刊

作者： Li, Xujiao 1 ; Tian, Jipeng 2 ; Zhang, Qing 3 ; Jiang, Yun 4 ; Wu, Zhe 1 ; Yu, Zhu 1 ;

作者机构： 1.China Agr Univ, Inst Grassland Sci, Beijing 100193, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Livestock Sci, Nanjing 210014, Jiangsu, Peoples R China

3.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China

4.Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA

关键词： proteolysis; silage; alfalfa; red clover

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2018 年 101 卷 10 期

页码：

收录情况： SCI

摘要： This study was conducted to study the effects of ensiled alfalfa (Medicago sativa) and red clover (Trifoliurn pratense) at different ratios on dynamics of fermentation parameters, N distribution, protein fractions, and protease activities during ensiling. Alfalfa and red clover were harvested and wilted to 35 and 25% dry matter, respectively, chopped to 1 cm, mixed, weighed into 1.0-L buckets at a density of 700 g/L, and ensiled for 1, 3, 7, 15, and 30 d at 30 degrees C. The treatments were mixing ratio of alfalfa to red clover at 100:0, 70:30, 50:50, 30:70, and 0:100 (RO, R30, R50, R70, and R100, respectively; fresh weight). For each ensiling duration, 3 replicates of each treatment were prepared. With increasing proportion of red clover in silage, total N content and proportions of nonprotein N, peptide N, free amino acid N, and NH3-N decreased linearly, and PC (indigestible true protein, acid detergent insoluble N) proportion increased linearly after ensiling. Moreover, the final pH was lower in R50 and R100 than RO (4.29, 4.20 vs. 4.48, respectively) on d 30. Also, lactic acid concentration on d 30 was higher in R50, R70, and R100 silage compared with RO (7.77, 7.66, and 8.76 vs. 6.34, % of dry matter, respectively). The proportion of NH 3 -N in R50 was lower than in RO but closer to R100 after ensiling. During ensiling, proteases including carboxypeptidase, aminopeptidase, and acid proteinase activities decreased as red clover proportion increased. However, no differences were detected in aminopeptidase and acid proteinase activities among R50, R70, and R100 during ensiling. Overall, 50:50 was the optimal mixing ratio of alfalfa with red clover, showing good fermentation quality with lower pH and higher lactic acid concentration, reduced protease activities and proteolysis compared with pure alfalfa silage, and also more total N content than pure red clover silage.

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