Genetic Diversity, Population Structure, and Botanical Variety of 320 Global Peanut Accessions Revealed Through Tunable Genotyping-by-Sequencing
文献类型: 外文期刊
作者: Zheng, Zheng 1 ; Sun, Ziqi 1 ; Fang, Yuanjin 1 ; Qi, Feiyan 1 ; Liu, Hua 1 ; Miao, Lijuan 1 ; Du, Pei 1 ; Shi, Lei 1 ; Gao, 1 ;
作者机构: 1.Henan Acad Agr Sci, Henan Prov Key Lab Genet Improvement Oil Crops, Minist Agr, Ind Crops Res Inst,Key Lab Oil Crops Huanghuaihai, Zhengzhou 450002, Henan, Peoples R China
2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
期刊名称:SCIENTIFIC REPORTS ( 影响因子:4.379; 五年影响因子:5.133 )
ISSN: 2045-2322
年卷期: 2018 年 8 卷
页码:
收录情况: SCI
摘要: Cultivated peanut (Arachis hypogaea L.) were classified into six botanical varieties according to the morphological characteristics. However, their genetic evolutionary relationships at the genome-wide level were still unclear. A total of 320 peanut accessions, including four of the six botanical varieties, and 37,128 high-quality single nucleotide polymorphisms (SNPs) detected by tunable genotyping by-sequencing (tGBS) were used to reveal the evolutionary relationships among different botanical varieties and verify the phenotypic classification. A phylogenetic tree indicated that the tested accessions were grouped into three clusters. Almost all of the peanut accessions in cluster C1 belong to var. fastigiata, and clusters C2 and C3 mainly consisted of accessions from var. vulgaris and subsp. hypogaea, respectively. The results of a principal component analysis were consistent with relationships revealed in the phylogenetic tree. Population structure analysis showed that var. fastigiata and var. vulgaris were not separated when K = 2 (subgroup number), whereas they were clearly divided when K = 3. However, var. hypogaea and var. hirsuta could not be distinguished from each other all the way. The nucleotide diversity (p) value implied that var. vulgaris exhibited the highest genetic diversity (0.048), followed by var. fastigiata (0.035) and subsp. hypogaea (0.012), which is consistent with the result of phylogenetic tree. Moreover, the fixation index (FST) value confirmed that var. fastigiata and var. vulgaris were closely related to each other (FST = 0.284), while both of them were clearly distinct from var. hypogaea (FST > 0.4). The present study confirmed the traditional botanical classifications of cultivated peanut at the genome-wide level. Furthermore, the reliable SNPs identified in this study may be a valuable resource for peanut breeders.
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