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Molecular characterization of a novel ovodefensin gene in chickens

文献类型： 外文期刊

作者： Yu, Lin-tian 1 ; Xiao, Ying-Ping 3 ; Li, Jing-Jing 1 ; Ran, Jin-Shan 1 ; Yin, Ling-Qian 1 ; Liu, Yi-Ping 1 ; Zhang, Lo 1 ;

作者机构： 1.Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, Chengdu Campus, Chengdu 611130, Sichuan, Peoples R China

2.China West Normal Univ, Inst Ecol, Nanchong 637009, Sichuan, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Qual & Stand Agraprod, Hangzhou 310021, Zhejiang, Peoples R China

4.Guangxi Agr Vocat Coll, Nanning 530007, Guangxi, Peoples R China

关键词： Ovodefensins; Host defense peptides; Phylogenetic analysis; Antimicrobial activity

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 678 卷

页码：

收录情况： SCI

摘要： Host defense peptides (HDPs) represent a large group of diverse small peptides that play important roles in host defense and disease resistance. In vertebrates, one of the main types of HDPs belong to defensins, which are less than 100 amino acid residues and characterized by a highly conserved motif of cysteine residues. Recently, a subfamily of defensins, namely ovodefensins (OvoDs), has been identified in birds and reptiles. However, both their family members and evolutionary relationships remain unclear. In the present study, we cloned and characterized a novel gene namely OvoDB beta in chickens. Our results showed that the full length of chicken OvoDB beta mRNA contains 344 bp nucleotides and encodes a 61-amino acid protein. We further revealed that the mRNA of OvoDB beta is abundant in the oviduct of laying hens but absent in many other tissues. Additionally, sequences comparison and analyses suggested that OvoDB beta is orthologous to the gene previously known as zebra finch OvoDB1, albeit it might exhibit specific structures. Furthermore, both OvoDB alpha and OvoDB beta were existent in the genome of each bird, implying that two types of OvoDBs sharing same cysteine motif have already emerged before the species divergence. More importantly, recombinant OvoDB beta mature peptide exerted antibacterial activity against Escherischia coli (CICC23657 strain) in vitro. These results collectively indicated that the putative sequence, namely chicken OvoDB beta, is a function gene with potential antimicrobial property. Discovery and function characterization of novel HDP genes may help us develop novel antimicrobial agents in the future.

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