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Genomic Identification and Expression Analysis of the Cathelicidin Gene Family of the Forest Musk Deer

文献类型： 外文期刊

作者： Zhang, Long 1 ; Jie, Hang 3 ; Xiao, Yingping 4 ; Zhou, Caiquan 1 ; Lyu, Wentao 4 ; Bai, Wenke 1 ;

作者机构： 1.China West Normal Univ, Minist Educ, Inst Ecol, Key Lab Southwest China Wildlife Resources Conser, Nanchong 637009, Peoples R China

2.China West Normal Univ, Sichuan Wildlife Rehabil & Breeding Res Ctr, Nanchong 637009, Peoples R China

3.Chongqing Inst Med Plant Cultivat, Chongqing Engn Technol Res Ctr GAP Genuine Med Ma, Nanchuan 408435, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Key Lab Qual & Safety Agroprod Prepared, Hangzhou 310021, Zhejiang, Peoples R China

关键词： cathelicidin; forest musk deer; phylogenetic analysis; expression pattern

期刊名称：ANIMALS （ 影响因子：2.752； 五年影响因子：2.942 ）

ISSN： 2076-2615

年卷期： 2019 年 9 卷 8 期

页码：

收录情况： SCI

摘要： Simple Summary Cathelicidins are a group of host defense peptides in vertebrates with both antimicrobial and immunomodulatory activities. In the present study, we identified the entire repertoire of the cathelicidin gene family from the forest musk deer genome. Sequence comparison, phylogenetic topology, and gene and genomic organizations collectively suggest that all cathelicidin genes have already been fixed in the genome of forest musk deer before the split of moschidae and bovidae, while independent pseudogenization events have occurred after species divergence. In addition, real-time PCR analysis suggested that all functional cathelicidins play important roles in the immune system. The results of this study will be helpful for further evolutionary and functional studies. The forest musk deer (Moschus berezovskii) is a small-sized artiodactyl species famous for the musk secreted by adult males. In the captive population, this species is under the threat of infection diseases, which greatly limits the increase of individual numbers. In the present study, we computationally analyzed the repertoire of the cathelicidin (CATHL) family from the genome of forest musk deer and investigated their expression pattern by real-time PCR. Our results showed that the entire genome of forest musk deer encodes eight cathelicidins, including six functional genes and two pseudogenes. Phylogenetic analyses further revealed that all forest musk deer cathelicidin members have emerged before the split of the forest musk deer and cattle and that forest musk deer CATHL3L2 and CATHL9 are orthologous with two cattle pseudogenes. In addition, the gene expression results showed that the six functional genes are not only abundantly expressed in the spleen and lung, but are also differently expressed in response to abscesses, which suggests that forest musk deer cathelicidins may be involved in infections. Taken together, identification and characterization of the forest musk deer cathelicidins provide fundamental data for further investigating their evolutionary process and biological functions.

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