文献类型： 外文期刊

作者： Tao, Shiyu ¹ ; Fan, Jinping ¹ ; Li, Jingjing ¹ ; Wu, Zhifeng ¹ ; Yao, Yong ¹ ; Wang, Zhenyu ² ; Wu, Yujun ² ; Liu, Xiangdong ¹ ; Xiao, Yingping ³ ; Wei, Hong ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

关键词： Diarrhea; Microbiome; Probiotics; Extracellular vesicles; Macrophage

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：13.0； 五年影响因子：11.6 ）

ISSN： 2090-1232

年卷期： 2025 年 69 卷

页码：

收录情况： SCI

摘要： Introduction: Diarrheic disease is a common intestinal health problem worldwide, causing great suffering to humans and animals. Precise manipulation strategies based on probiotics to combat diarrheic diseases have not been fully developed. Objectives: The aim of this study was to investigate the molecular mechanisms by which probiotics manipulate macrophage against diarrheic disease. Methods: Metagenome reveals gut microbiome profiles of healthy and diarrheic piglets. Fecal microbial transplantation (FMT) was employed to explore the causal relationship between gut microbes and diarrhea. The protective role of probiotics and their derived extracellular vesicles (EVs) was investigated in ETEC K88-infected mice. Macrophage depletion was performed to assess the role of macrophages in EVs against diarrhea. Execution of in vitro cell co-culture and transcriptome analyses elucidated the molecular mechanisms by which EVs modulate the macrophage and intestinal epithelial barrier. Results: Escherichia coli was enriched in weaned diarrheic piglets, while Lactobacillus johnsonii (L. john) showed a negative correlation with Escherichia coli. The transmission of diarrheic illness symptoms was achieved by transferring fecal microbiota, but not metabolites, from diarrheic pigs to germ-free (GF) mice. L. john's intervention prevented the transmission of disease phenotypes from diarrheic piglets to GF mice. L. john also reduces the gut inflammation induced by ETEC K88. The EVs secreted by L. john demonstrated enhanced efficacy in mitigating the adverse impacts induced by ETEC K88 through the modulation of macrophage phenotype. In vitro experiments have revealed that EVs activate M2 macrophages in a manner that shuts down ERK, thereby inhibiting NLRP3 activation in intestinal epithelial cells. Conclusion: Our results reveal that intestinal microbiota drives the onset of diarrheic disease and that probiotic-derived EVs ameliorate diarrheic disease symptoms by modulating macrophage phenotypes. These findings can enhance the advancement of innovative therapeutic approaches for diarrheic conditions based on probiotic-derived EVs. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).