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Transcriptome characterization and gene expression analysis related to chemoreception in Trichogramma chilonis, an egg parasitoid

文献类型： 外文期刊

作者： Liu, Jian-Bai 1 ; Wu, Han 1 ; Yi, Jie-Qun 1 ; Song, Zi-Wei 2 ; Li, Dun-Song 2 ; Zhang, Gu-Ren 1 ;

作者机构： 1.Sun Yat Sen Univ, Inst Entomol, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Technol Plant Protect, Plant Protect Res Inst, Guangzhou 510640, Guangdong, Peoples R China

关键词： Transcriptomic analysis; Chemosensory gene; Trichogramma chilonis; qRT-PCR

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 678 卷

页码：

收录情况： SCI

摘要： Chemoreception is critical for the survival of insects. Insects have a variety of behavioral responses, such as mating, host searching and ovipositing, in response to different odor signals detected in their living environment. Trichogramma chilonis, an egg parasitoid, acts as an efficient and effective biocontrol reagent for many agricultural and forestry insect pests in many parts of China. However, little is known about the molecular mechanism of the olfaction-evoked behavior in T. chilonis. In the present study, we conducted transcriptome profiling analysis of T. chilonis based on the Illumina high-throughput sequencing platform in order to explore differences of chemoreception between male and female T. chilonis. In this study, a total of 85 chemosensory genes were identified from transcriptomic data, including 45 odorant receptors (ORs), 22 odorant binding proteins (OBPs), 14 ionotropic receptors (IRs), 2 sensory neuron membrane proteins (SNMPs) and 2 chemosensory proteins (CSPs). From the analysis of the transcriptome, most of the candidate olfactory genes had similar expression levels in males and females, including a few OR and OBP genes (TchiOR38, TchiOR39, TchiOR40, TchiOE41, TchiOR42, TchiOR43, TchiOR44, TchiOR45, TchiOBP1, TchiOBP4, TchiOBP10, TchiOBP12, TchiOBP18 and TchiOBP19) which showed male-biased expression. Some annotated unigenes were chosen randomly to have qRT-PCR, which verified the correctness of analysis of transcriptome in T. chilonis. This is the first study to obtain and identify candidate genes related to chemoreception in T. chilonis. Our work lays a solid foundation for related future research on the chemosensory system of T. chilonis at the molecular level and helps advance the use of T. chilonis as biological control agents.

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