Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading Rhodococcus erythropolis D-1
文献类型： 外文期刊
作者： Tao, Yang 1 ; Wang, Wen-Guang 1 ; Lu, Sheng-Hui 1 ; Xie, Xin 1 ; Lazzat, Ugit 1 ; Bai, Naling 2 ; Zhao, Yuhua 1 ; Jiang, 1 ;
作者机构： 1.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Ecoenvironm Protect Inst, Shanghai 201403, Peoples R China
关键词： Rhodococcus erythropolis; Genetic manipulation; Ribosome binding site
期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）
ISSN： 1590-4261
年卷期： 2018 年 68 卷 11 期
页码：
收录情况： SCI
摘要： Rhodococcus strains not only have been widely used in industries but also have a potential ability of producing new structural natural products. Integration of heterologous genes into chromosomes of Rhodococcus strains for gene expression can facilitate the studies and applications of these strains. A conjugation system was optimized in order to transfer enhanced green fluorescent protein (EGFP) encoding gene as a reporter from Escherichia coli into Rhodococcus erythropolis D-1. The influence of three native ribosome binding sites (RBSs) and two designed RBSs on the target protein production in R. erythropolis D-1 was also characterized. An efficient conjugation system of R. erythropolis D-1 was established to integrate EGFP gene into its chromosome. Among of five RBSs, RBS3 showed the highest translational activity in R. erythropolis D-1.
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