Development of a VP38 recombinant protein-based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II)
文献类型： 外文期刊
作者： Zeng, Weiwei 1 ; Wang, Yingying 1 ; Guo, Yanmin; Bergmann, Sven M.; Yin, Jiyuan 1 ; Li, Yingying 1 ; Ren, Yan 1 ; S 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou, Guangdong, Peoples R China
2.Chinese Ac
关键词： ELISA; GCRV; immunoglobulin M; monoclonal antibody; VP38
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）
ISSN： 0140-7775
年卷期： 2018 年 41 卷 12 期
页码：
收录情况： SCI
摘要： Currently, serological assays for grass carp reovirus genotype II (GCRV-II) diagnosis are not available. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against GCRV-II was developed. The structural protein VP38 of GCRV-II was used as the coating antigen. Monoclonal antibodies (mAb) against IgM of grass carp labelled with HRP were used as a secondary antibody. The antigen concentration and serum dilution were optimized using chess board titration. Furthermore, the specificity of indirect ELISA assay was confirmed by cross check with sera positive for other grass carp pathogens. In comparison with results obtained from indirect immunofluorescence assay (IFA) and Western blot by testing of 60 serum samples to evaluate the sensitivity and specificity of the ELISA, agreement between 90% and 96.7% was reached, respectively. A serological survey was performed using the assay with grass carp field serum samples. The seropositive rate of the 242 serum samples was 69.8%. In conclusion, the developed indirect ELISA is a very specific and sensitive test that will be useful for large-scale serological surveys to detect indirectly GCRV II infections as well as to monitor the changes of antibody level after immunization.
- 相关文献
作者其他论文 更多>>
-
Identification of a novel strain of carp edema virus affecting Songpu mirror carp in Liaoning, China
作者：Shi, Wen;Han, Bing;He, Huilin;Feng, Ruixue;Guo, Siyi;Ren, Xuanyu;Huang, Jinshan;Lin, Xu;Liu, Min;Yin, Jiyuan;Lv, Xiaonan
关键词：Carp edema virus disease; Carp edema virus; Detection; Songpu mirror carp; Genotype IIa
-
Cyprinid herpesvirus 3 replication is inhibited via decreased ORF3 promoter activity mediated by multiple cellular transcription factors
作者：Liu, Donghai;Wang, Yingying;Li, Yingying;Mo, Xubing;Yin, Jiyuan;Wang, Qing;Zheng, Shucheng;Liu, Donghai
关键词：Cyprinid herpesvirus 3; Koi herpesvirus; ORF3; Promoter; Transcription factors
-
Identification of a novel birnavirus strain in diseased Siniperca chuatsi and transcriptome profiling of virus-infected MFSB cells
作者：Cui, Hongye;Chen, Haiyue;Wang, Yuhui;Wang, Yaoda;Hu, Tianmei;Wu, Baozhou;Hao, Shuguang;Zhang, Jiping;Liu, Yu;Zeng, Weiwei;Xu, Liwen;Hu, Xiong
关键词：Siniperca chuatsi; Birnavirus; Genome sequence; SCBV; Immune response
-
Enhanced virulence of Acinetobacter johnsonii at low temperatures induces acute immune response and systemic infection in American bullfrogs (Aquarana catesbeiana)
作者：Lin, Han;Sun, Jingyang;Li, Hua;Chen, Kesong;Qin, Zhendong;Jiang, Biao;Li, Wei;Su, Youlu;Lin, Li;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Cold stress; Virulence genes; Amphibian disease; Immune response; Systemic infection
-
Methyltransferase HsdM Regulates the Pathogenicity of Streptococcus agalactiae to Nile Tilapia (Oreochromis niloticus)
作者：Jiang, Dongdong;Yu, Meiling;Jiang, Dongdong;Wang, Bei;Jiang, Dongdong;Ren, Yan;Mo, Xubing;Zhang, Defeng
关键词：
Streptococcus agalactiae ; DNA methyltransferase; adhesion; pathogenesis; growth rate; Nile tilapia -
Frog virus 3: Prevalence survey and quantitative analysis in American bullfrogs using TaqMan MGB probe real-time fluorescence quantitative PCR
作者：Li, Hua;Liu, Xiaobing;Sun, Jingyang;Lin, Han;Chen, Kesong;Hu, Yueming;Jiang, Mengzhen;Jiang, Biao;Li, Wei;Su, Youlu;Liu, Chun;Wang, Qing;Wang, Qing
关键词：Aquarana catesbeiana; Frog virus 3; TaqMan-MGB real-time PCR; Virus load; Prevalence survey
-
Enhanced stability and rheological properties of myofibrillar proteins emulsions conferred by oat β-glucan: Insights into structural and interfacial interactions
作者：Wu, Yanyan;Wu, Yamei;Zhao, Yongqiang;Xiang, Huan;Wang, Yueqi;Wu, Yanyan;Zhao, Yongqiang;Wang, Yueqi;Wu, Yamei;Hao, Zhiming;Wang, Qing
关键词：Oat beta-glucan; Myofibrillar protein; Conformational features; Interfacial property; Rheological property
