Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion
文献类型： 外文期刊
作者： Yu, Yanfei 1 ; Liu, Maojun 1 ; Hua, Lizhong 1 ; Qiu, Mingjun 1 ; Zhang, Wei 4 ; Wei, Yanna 1 ; Gan, Yuan 1 ; Feng, Zhixin 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China
2.State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing, Jiangsu, Peoples R China
3.Shanxi Agr Univ, Coll Anim Sci & Technol, Taigu, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Minist Agr, Key Lab Anim Bacteriol, Nanjing, Jiangsu, Peoples R China
期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）
ISSN： 0928-4249
年卷期： 2018 年 49 卷
页码：
收录情况： SCI
摘要： Mycoplasma hyopneumoniae is an important respiratory pathogen that causes great economic losses to the pig industry worldwide. Although some putative virulence factors have been reported, pathogenesis remains poorly understood. Herein, we evaluated the relative abundance of proteins in virulent 168 (F107) and attenuated 168L (F380) M. hyopneumoniae strains to identify virulence-associated factors by two-dimensional electrophoresis (2-DE). Seven proteins were found to be 1.5-fold more abundant in 168, and protein-protein interaction network analysis revealed that all seven interact with putative virulence factors. Unexpectedly, six of these virulence-associated proteins are encoded by core rather than accessory genomic elements. The most differentially abundant of the seven, fructose-1,6-bisphosphate aldolase (FBA), was successfully cloned, expressed and purified. Flow cytometry demonstrated the surface localisation of FBA, recombinant FBA (rFBA) mediated adhesion to swine tracheal epithelial cells (STEC), and anti-rFBA sera decreased adherence to STEC. Surface plasmon resonance showed that rFBA bound to fibronectin with a moderately strong K-D of 469nM. The results demonstrate that core gene expression contributes to adhesion and virulence in M. hyopneumoniae, and FBA moonlights as an important adhesin, mediating binding to host cells via fibronectin.
- 相关文献
作者其他论文 更多>>
-
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者：Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词：
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者：Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词：Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
A genome-wide association analysis for salt tolerance during the soybean germination stage and development of KASP markers
作者：Wang, Junyan;Zhou, Miaomiao;Su, Chengfu;Wang, Junyan;Zhou, Miaomiao;Zhang, Hongmei;Liu, Xiaoqing;Zhang, Wei;Wang, Qiong;Jia, Qianru;Chen, Huatao;Xu, Donghe;Chen, Huatao
关键词：soybean; salt tolerance; germination stage; genome-wide association analysis; KASP marker
-
Therapeutic efficacy of a K5-specific phage and depolymerase against Klebsiella pneumoniae in a mouse model of infection
作者：Li, Pei;Zheng, Xiangkuan;Xu, Sixiang;Zhou, Yu;Qin, Xiayan;Hu, Zimeng;Ma, Jiale;Zhang, Wei;Li, Pei;Zheng, Xiangkuan;Xu, Sixiang;Zhou, Yu;Hu, Zimeng;Zhang, Wei;Guo, Genglin;Yu, Yanfei;Tan, Zhongming;Chen, Long
关键词：Klebsiella pneumoniae; phage; depolymerase; K5; hypervirulent; capsule
-
Selected Electrosynthesis of 3-Aminopyrazoles from α,β-Alkynic Hydrazones and Secondary Amines
作者：Tang, Dong;Zhang, Wei;Ji, Jingwen;Jiang, Rui;Wan, Yaya;Zhou, Pengjuan;Ma, Wei
关键词：
-
Genome-Wide Association Study on Candidate Genes Associated with Soybean Stem Pubescence and Hilum Colors
作者：Zhou, Miaomiao;Wang, Junyan;Su, Chengfu;Chen, Huatao;Wang, Qiong;Chen, Huatao;Hu, Shengyan;Xiong, Yawen;Chen, Huatao;Jia, Qianru;Zhang, Hongmei;Zhang, Wei;Wang, Qiong
关键词：soybean; stem pubescence color; hilum color; genome-wide association study; SNP; candidate gene
-
Genome-Wide Association Study and Identification of Candidate Genes Associated with Seed Number per Pod in Soybean
作者：Wang, Qiong;Zhang, Wei;Xu, Wenjing;Zhang, Hongmei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Chen, Huatao
关键词：soybean; seed number per pod; leaf characteristics; genome-wide association study; single nucleotide polymorphism
