Generation of panels of anti-idiotypic single-chain variable fragments mimicking Cry2Aa toxin using the chain shuffling technique
文献类型： 外文期刊
作者： Liu, Yuan 1 ; Lin, Manman 1 ; Wu, Jiaxin 1 ; Hu, Xiaodan 1 ; Zhang, Xiao 1 ; Xu, Chongxin 1 ; Zhong, Jianfeng 1 ; Xie, Ya 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing, Jiangsu, Peoples R China
2.Huaiyin Inst Technol, Huaian, Peoples R Chin
关键词： Chain shuffling; anti-idiotypic antibodies; single-chain variable fragments (scFvs); Cry2Aa toxin
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN： 0954-0105
年卷期： 2018 年 29 卷 1 期
页码：
收录情况： SCI
摘要： Here, we firstly reported to generate panels of anti-idiotypic singlechain variable fragments (scFvs) of Bacillus thuringiensis Cry2Aa toxin by chain shuffling method. Light-and heavy-chain-shuffled libraries based on two parental clones were constructed. The signal of the light-chain-shuffled library was 4.2-fold higher than the heavy one. Solely after one round panning, eight mutants were rapidly isolated from the chain-shuffled libraries. The apparent affinity of the best clones was 2.65 x 10(6) M-1, a 2.59-fold improvement over the parental clone. The binding abilities between anti-idiotypic scFvs with brush border membrane vesicle of Plutella xylostella larvae were also determined. This work demonstrated that chain shuffling could rapidly generate panels of antibodies with similar binding abilities but of the distinct idiotypic composition. The serials of mutants can be used to map the binding epitope of the receptors in target insects instead of Cry2Aa toxin.
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