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Development of novel microsatellites for population genetic analysis of Phenacoccus solenopsis Tinsley (Hemipeta: Pseudoccoccidae) based on genomic analysis

文献类型： 外文期刊

作者： Ma, Ling 2 ; Cao, Li-Jun 3 ; Gong, Ya-Jun 3 ; Hoffmann, Ary A. 4 ; Zeng, Ai-Ping 1 ; Wei, Shu-Jun 3 ; Zhou, Zhong-Shi 2 ;

作者机构： 1.Hunan Agr Univ, Inst Insect Sci, Changsha 410128, Hunan, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

4.Univ Melbourne, Inst Bio21, Sch BioSci, Melbourne, Vic 3010, Australia

关键词： Phenacoccus solenopsis; Genome; Microsatellites

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2019 年 121 卷

页码：

收录情况： SCI

摘要： The cotton mealybug, Phenacoccus solenopsis Tinsley (Hemipeta: Pseudoccoccidae), is an aggressively invasive pest causing huge economic losses of crops around the world. In this study, we developed genome-wide microsatellites for population genetic analysis of P. solenopsis. We obtained a random genome of P. solenopsis with a size of 267.07 Mb and scaffold N50 of 14.12 Kb. In total 115,639 microsatellites were isolated from the genome, of which those with trinucleotide motifs were the most abundant. Forty-two polymorphic loci were selected for primer validation based on three populations. Allele numbers varied from 2 to 5 with an average value of 2.5 per locus, and allelic richness ranged from 1.00 to 4.48. The observed heterozygosity (H-0) and expected heterozygosity (H-E) ranged from 0.00 to 0.92 and 0.00 to 0.73, respectively. Population genetic structure analysis based on the developed markers revealed strong differentiation between three populations of P. solenopsis collected from its invasive range in China. The microsatellites developed in our study should provide efficient genetic markers for population level studies of P. solenopsis to reveal invasion history and patterns of dispersal. (C) 2018 Published by Elsevier B.V.

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