An efficient method for immature embryo rescue and plant regeneration from the Calli of Ziziphus jujuba 'Lengbaiyu'
文献类型： 外文期刊
作者： Ren, Haiyan 1 ; Du, Xuemei 2 ; Li, Dengke 2 ; Zhao, Ailing 2 ; Wang, Yongkang 2 ; Xue, Xiaofang 2 ; Gong, Guihua 2 ; Du, 1 ;
作者机构： 1.Shanxi Agr Univ, Sch Hort, Mingxian Rd 1, Taigu 030801, Shanxi, Peoples R China
2.Shanxi Acad Agr Sci, Inst Pomol, Shanxi Prov Key Lab Genet Improvement & Use Pomol, Taigu, Peoples R China
关键词： culture technique; immature embryo rescue; callus culture; seedlings; Chinese jujube
期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）
ISSN： 1462-0316
年卷期： 2019 年 94 卷 1 期
页码：
收录情况： SCI
摘要： Hybrid breeding of Chinese jujube (Ziziphus jujube) is limited by embryo abortion. Improving the success of embryogenic progeny and immature embryo culture techniques could increase the breeding efficiency of new jujube varieties. We optimised the current immature embryo culture techniques by using immature embryos before the globular stage of Chinese jujube variety Lengbaiyu. The optimal growth media were as follows: for 30-day-old immature embryo growth, MS + IBA 0.8 mg center dot L-1 + ZT 0.5 mg center dot L-1 + GA(3) 7.0 mg center dot L-1 + NAA 0.2 mg center dot L-1 + LH 0.5 mg center dot L-1 + 5% sucrose, with 22.22% embryonic growth rate and 0.41% plantlet formation rate; and for young embryo differentiation, MS + TDZ (0.8 mg center dot L-1) + IAA (0.5 mg center dot L-1) + sucrose (3%), with 81.12% differentiation rate. Thus, regeneration via callus induction effectively improved the efficiency of embryo rescue from young immature embryos in Lengbaiyu, which might provide new approaches for breeding Chinese jujube.
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