文献类型： 外文期刊

作者： Gu, Qiongnan ¹ ; Yuan, Qinfeng ¹ ; Zhao, Dian ¹ ; Huang, Junbin ¹ ; Hsiang, Tom ³ ; Wei, Yangdou ⁴ ; Zheng, Lu ¹ ;

作者机构： 1.Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei, Peoples R China

2.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Key Lab Integrated Pest Management Crops Cent Chi, Minist Agr,Hubei Key Lab Crop Dis Insect Pests &, Wuhan 430064, Hubei, Peoples R China

3.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

4.Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada

关键词： carbon metabolism; ChAcs; Colletotrichum higginsianum; lipid metabolism; plant infection

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2019 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Acetyl-coenzyme A (acetyl-CoA) is a key molecule that participates in many biochemical reactions in amino acid, protein, carbohydrate and lipid metabolism. Here, we genetically dissected the distinct roles of two acetyl-CoA synthetase genes, ChAcs1 and ChAcs2, in the regulation of fermentation, lipid metabolism and virulence of the hemibiotrophic fungus Colletotrichum higginsianum. ChAcs1 and ChAcs2 are both highly expressed during appressorial development and the formation of primary hyphae, and are constitutively expressed in the cytoplasm throughout development. We found that C. higginsianum strains without ChAcs1 were non-viable in the presence of most non-fermentable carbon sources, including acetate, ethanol and acetaldehyde. Deletion of ChAcs1 also led to a decrease in lipid content of mycelia and delayed lipid mobilization in conidia to developing appressoria, which suggested that ChAcs1 contributes to lipid metabolism in C. higginsianum. Furthermore, a ChAcs1 deletion mutant was defective in the switch to invasive growth, which may have been directly responsible for its reduced virulence. Transcriptomic analysis and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) revealed that ChAcs1 can affect the expression of genes involved in virulence and carbon metabolism, and that plant defence genes are up-regulated, all demonstrated during infection by a ChAcs1 deletion mutant. In contrast, deletion of ChAcs2 only conferred a slight delay in lipid mobilization, although it was highly expressed in infection stages. Our studies provide evidence for ChAcs1 as a key regulator governing lipid metabolism, carbon source utilization and virulence of this hemibiotrophic fungus.