Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3
文献类型: 外文期刊
作者: Ma Dong-fang 1 ; Hou Lu 2 ; Sun Cai 2 ; Zhang Xing 2 ; Yin Jun-liang 2 ; Guo Qing-yun 1 ; Zhu Yong-xing 2 ;
作者机构: 1.Qinghai Univ, Key Lab Agr Integrated Pest Management, Qinghai Prov State Key Lab Plateau Ecol & Agr, Qinghai Acad Agr & Forestry Sci, Xining 810016, Qinghai, Peoples R China
2.Yangtze Univ, Coll Agr, Hubei Collaborat Innocvt Ctr Grain Ind, Jingzhou 434023, Peoples R China
关键词: Puccinia striiformis; Psathyrostachys huashanica; stripe rust; molecular mapping; resistance gene
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2019 年 18 卷 1 期
页码:
收录情况: SCI
摘要: Several new stripe rust pathogen races emerged in the wheat growing regions of China in recent years. These races were virulent to most of the designated wheat seedling resistance genes. Thus, it is necessary and worthwhile to identify new valuable resistant materials for the sake of diversifying resistant sources, pyramiding different resistance genes and achieving durable resistance. Here, we identified the resistance gene, temporarily designated as YrH9017, in wheat-Psathyrostachys huashanica introgression line H9017-14-16-5-3. A total of 146 F-2 plants and their derived F-2:3 families in a cross of Mingxian 169 and H9017-14-16-5-3 were used to evaluate seedling stripe rust response and as a mapping population. Finally, we constructed a genetic map including eight simple sequence repeat (SSR) markers and expressed sequence tag (EST) markers. YrH9017 was located on the long arm of chromosome 2A and closely linked with two EST-sequence tagged site (EST-STS) markers BG604577 and BE471201 at 1.3 and 1.8 cM distance, respectively. The two closest markers could be used for marker-assisted selection of YrH9017 in breeding.
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关键词:Haynaldia villosa;translocation line;stripe rust;RGAP-SSR;molecular mapping