文献类型： 外文期刊

作者： Liu, Tengfei ¹ ; Liu, Zhi ¹ ; Fan, Jingwei ¹ ; Yuan, Yaqin ¹ ; Liu, Haiyue ² ; Xian, Wenfei ³ ; Xiang, Shuaiying ¹ ; Yang, Xia ¹ ; Liu, Yucheng ¹ ; Liu, Shulin ¹ ; Zhang, Min ¹ ; Jiao, Yuannian ⁴ ; Cheng, Shifeng ³ ; Doyle, Jeff J. ⁵ ; Xie, Fang ² ; Li, Jiayang ⁶ ; Tian, Zhixi ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Seed Innovat, Beijing, Peoples R China

2.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Carbon Capture, Shanghai, Peoples R China

3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr,Shenzhen Branch, Shenzhen, Peoples R China

4.Chinese Acad Sci, Inst Bot, State Key Lab Plant Divers & Specialty Crops, Beijing, Peoples R China

5.Cornell Univ, Sch Integrat Plant Sci, Sect Plant Biol & Plant Breeding & Genet, Ithaca, NY 14850 USA

6.Chinese Acad Sci, State Key Lab Plant Genom, Beijing, Peoples R China

7.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing, Peoples R China

8.Univ Chinese Acad Sci, Beijing, Peoples R China

9.Max Planck Inst Biol Tubingen, Dept Mol Biol, Tubingen, Germany

10.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Hebei Key Lab Crop Genet & Breeding, Shijiazhuang, Peoples R China

11.Yazhouwan Natl Lab, Sanya, Hainan, Peoples R China

关键词： Evolution of root nodule symbiosis; Leguminosae; Phylogenomic analysis; Gene loss; Lateral suppressor

期刊名称：GENOME BIOLOGY （ 影响因子：9.4； 五年影响因子：16.3 ）

ISSN： 1474-760X

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Background: Root nodule symbiosis (RNS) is a fascinating evolutionary event. Given that limited genes conferring the evolution of RNS in Leguminosae have been functionally validated, the genetic basis of the evolution of RNS remains largely unknown. Identifying the genes involved in the evolution of RNS will help to reveal the mystery. Results: Here, we investigate the gene loss event during the evolution of RNS in Leguminosae through phylogenomic and synteny analyses in 48 species including 16 Leguminosae species. We reveal that loss of the Lateral suppressor gene, a member of the GRAS-domain protein family, is associated with the evolution of RNS in Leguminosae. Ectopic expression of the Lateral suppressor (Ls) gene from tomato and its homolog MONOCULM 1 (MOC1) and Os7 from rice in soybean and Medicago truncatula result in almost completely lost nodulation capability. Further investigation shows that Lateral suppressor protein, Ls, MOC1, and Os7 might function through an interaction with NODULATION SIGNALING PATHWAY 2 (NSP2) and CYCLOPS to repress the transcription of NODULE INCEPTION (NIN) to inhibit the nodulation in Leguminosae. Additionally, we find that the cathepsin H (CTSH), a conserved protein, could interact with Lateral suppressor protein, Ls, MOC1, and Os7 and affect the nodulation. Conclusions: This study sheds light on uncovering the genetic basis of the evolution of RNS in Leguminosae and suggests that gene loss plays an essential role.