Response Surface Methodology for the Optimization of Ultrasound-Assisted Extraction of Tetrodotoxin from the Liver of Takifugu pseudommus
文献类型： 外文期刊
作者： Zhang, Xiaojun 1 ; Han, Chengcheng 2 ; Chen, Si 1 ; Li, Le 3 ; Zong, Jingjing 2 ; Zeng, Junjie 1 ; Mei, Guangming 1 ;
作者机构： 1.Marine Fisheries Res Inst Zhejiang, Lab Aquat Prod Proc & Qual Safety, Zhoushan 316100, Peoples R China
2.Zhejiang Ocean Univ, Sch Food & Pharm, Zhoushan 316022, Peoples R China
3.Chinese Acad Fishery Sci, Qual & Stand Res Ctr, Beijing 100141, Peoples R China
关键词： tetrodotoxin; response surface methodology; pufferfish
期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）
ISSN： 2072-6651
年卷期： 2018 年 10 卷 12 期
页码：
收录情况： SCI
摘要： Tetrodotoxin (TTX) is a marine biotoxin that has high scientific value. However, the lack of efficient TTX extraction and preparation methods has led to a scarcity of TTX samples for clinical application. In this study, TTX from the liver of Takifugu pseudommus was ultrasound-assisted extracted with acidified organic solvents. The extraction process was analyzed and optimized by single factor method and response surface methodology (RSM). The optimal extraction conditions predicted by a response surface model were as follows: liquid:material ratio, 2.8:1; extraction temperature, 60 degrees C; extraction time, 23.3 min. Under these conditions, the extraction of TTX had a yield of 89.65%, and the results were further verified by experimental extraction, and analyzed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). It was found that the extracts of T. pseudommus liver contained TTX and its four analogues at certain proportions (TTX: 10.4%; 5,6,11-trideoxyTTX: 83.3%; 5,11-dideoxyTTX:2.4%; 4,9-anhydro TTX:2.6%; 5-deoxyTTX:1.3%). This study demonstrates a stable and efficient extraction process of TTX from pufferfish liver, which can be helpful for further research and analysis, as well as the utilization of TTX from pufferfish.
- 相关文献
作者其他论文 更多>>
-
Exploring molecular mechanisms underlying changes in lipid fingerprinting of salmon (Salmo salar) during air frying integrating machine learning-guided REIMS and lipidomics analysis
作者：Song, Gongshuai;Zeng, Mingwei;Jiang, Nengliang;Wang, Danli;Yuan, Tinglan;Li, Ling;Gong, Jinyan;Song, Gongshuai;Chen, Shengjun;Song, Gongshuai;Shen, Qing;Lyu, Zhangfan;Mei, Guangming
关键词：Salmon; Air frying; Lipids oxidation; Lipidomics; Machine learning
-
Simultaneous removal of phosphate and tetracycline using LaFeO3 functionalised magnetic biochar by obtained ultrasound-assisted sol-gel pyrolysis: Mechanisms and characterisation
作者：Xu, Zhichao;Guo, Hongyang;Ahmed, Taosif;Wang, Teng;Liu, Jingxin;Mei, Meng;Chen, Si;Li, Jinping;Gan, Jinhua;Wang, Teng;Liu, Jingxin;Mei, Meng;Chen, Si;Li, Jinping
关键词：Bimetallic modification; Crab shell; Biochar adsorbent; Tetracycline and phosphate adsorption
-
Effects of effective microorganisms on the physiological status, intestinal microbiome, and serum metabolites of Eriocheir sinensis
作者：Li, Quanjie;Sun, Yi;Nie, Zhijuan;Du, Jinliang;Cao, Liping;Gao, Jiancao;Xu, Gangchun;Yi, Xiangyu;Li, Le;Xu, Gangchun
关键词：Eriocheir sinensis; Intestinal microbiome; Metabolites; Antioxidant capacity; Effective microorganisms
-
Effects of fattening diets on the nutritional quality and flavor of the adult female Chinese mitten crab (Eriocheir sinensis)
作者：Yi, Xiangyu;Li, Le;Zhang, Xing;Xu, Gangchun;Gao, Jiancao;Du, Jinliang;Nie, Zhijuan;Xu, Gangchun
关键词：Eriocheir sinensis; Fattening period; Diet combinations; Nutritional quality; Flavor -enhancing nucleotides
-
Study of the Quality and Nutritional Value of Alosa sapidissima in the Postmortem Process
作者：Li, Le;Yi, Xiangyu;Xu, Pao;Xu, Gangchun;Zhu, Haojun;Nie, Zhijuan;Zheng, Yao;Xu, Pao;Xu, Gangchun;Yang, Xiwei;Yu, Yaqing
关键词：Alosa sapidissima; postmortem; fish muscle; nutritional value; free amino acids; electronic tongue; quality changes
-
Simple sequence repeats drive genome plasticity and promote adaptive evolution in penaeid shrimp
作者：Yuan, Jianbo;Zhang, Xiaojun;Liu, Chengzhang;Li, Shihao;Yu, Yang;Gao, Yi;Liu, Fei;Zhang, Xiaoxi;Zhang, Chengsong;Xiang, Jianhai;Li, Fuhua;Yuan, Jianbo;Zhang, Xiaojun;Liu, Chengzhang;Li, Shihao;Yu, Yang;Gao, Yi;Liu, Fei;Zhang, Chengsong;Xiang, Jianhai;Li, Fuhua;Yuan, Jianbo;Zhang, Xiaojun;Liu, Chengzhang;Li, Shihao;Yu, Yang;Gao, Yi;Liu, Fei;Zhang, Chengsong;Xiang, Jianhai;Li, Fuhua;Wang, Min;Feng, Lu;Wang, Min;Feng, Lu;Sun, Yamin;Kong, Jie;Fan, Guangyi
关键词：
-
Accumulation and Elimination of Tetrodotoxin in the PufferfishTakifugu obscurusby Dietary Administration of the Wild Toxic GastropodNassarius semiplicata
作者：Zhang, Xiaojun;Chen, Si;Xu, Hanxiang;Zhang, Xiaojun;Chen, Si;Zong, Jingjing;Lu, Yibo;Wang, Ruirui;Xu, Hanxiang;Li, Menglong
关键词：tetrodotoxin; Takifugu obscurus; accumulation; elimination; Nassarius semiplicata
