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Metabolic Activity of Cytochrome P450s Towards Four Pyrethroids in Midgut Tissue From Locusta migratoria (Orthoptera: Acrididae)

文献类型： 外文期刊

作者： Zhu, Wenya 1 ; Zhang, Xueyao 1 ; Wu, Haihua 1 ; Liu, Jiao 1 ; Zhu, Kun Yan 5 ; Zhang, Jianzhen 1 ; Ma, Enbo 1 ;

作者机构： 1.Shanxi Univ, Res Inst Appl Biol, Taiyuan 030006, Shanxi, Peoples R China

2.Shanxi Univ, Coll Life Sci, Taiyuan 030006, Shanxi, Peoples R China

3.Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan 030031, Shanxi, Peoples R China

4.Lab Integrated Pest Management, Taiyuan 030031, Shanxi, Peoples R China

5.Kansas Stat

关键词： cytochrome P450 monooxygenase; pyrethroid; detoxification; Locusta migratoria

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN： 0022-0493

年卷期： 2018 年 111 卷 6 期

页码：

收录情况： SCI

摘要： Cytochrome P450 monooxygenases (P450s) play important roles in metabolizing various insecticides and often contribute to the development of insecticide resistance in insects and other arthropod species. The objective of this study was to compare the metabolism of four commonly used pyrethroids including deltamethrin, fluvalinate, fenvalerate, and permethrin in the midgut tissue of Locusta migratoria Linnaeus (Orthoptera: Acrididae) by using synergism bioassay and ultra-performance liquid chromatography (UPLC)-mass spectrometer (MS) analyses. Our study showed that piperonyl butoxide (PBO, P450 enzyme inhibitor) can significantly synergize the toxicity of deltamethrin, fluvalinate, and fenvalerate with synergism ratios ranging from 1.30 to 1.70 folds. Preincubations of the midgut tissue with PBO followed by incubations with each of the four pyrethroids resulted in significantly higher amounts of unmetabolized deltamethrin and fluvalinate than those in the control (preincubation without PBO) as well as preincubations with other two detoxification enzyme inhibitors. These results indicate that P450s play important roles in metabolizing deltamethrin and fluvalinate in the midgut tissue. Our further study using deltamethrin as a representative pyrethroid and UPLC-MS techniques confirmed that the reduced amount of deltamethrin in the control (preincubation without PBO) was due to the metabolism of deltamethrin to yield hydroxydeltamethrin which is a major metabolite produced by P450-mediated aromatic hydroxylation of deltamethrim. These results provide new insights into differential metabolic activity of P450s towards different pyrethroids in the midgut tissue of L. migratoria.

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