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In Vitro Formation of Dickeya zeae MS1 Biofilm

文献类型： 外文期刊

作者： Huang, Ning 1 ; Pu, Xiaoming 2 ; Zhang, Jingxin 2 ; Shen, Huifang 2 ; Yang, Qiyun 2 ; Wang, Zhongwen 1 ; Lin, Birun 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, 100 Daxue Rd, Nanning 530004, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Guangdong Key Lab High Technol Plant Protect, Guangzhou, Guangdong, Peoples R China

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2019 年 76 卷 1 期

页码：

收录情况： SCI

摘要： Bacterial soft rot caused by Dickeya zeae MS1 (Erwinia chrysanthemi) is one of the most devastating banana diseases worldwide. However, knowledge of the development and ecological interactions of D. zeae MS1 biofilm is limited. Here, we visualized the development and architecture of D. zeae MS1 biofilm using confocal laser scanning microscopy, and we evaluated the ability of D. zeae MS1 to form biofilms under different environmental conditions (carbon sources, temperatures, pH levels and mineral elements) using a microtiter plate assay. We found that the development of D. zeae MS1 biofilm could be categorized into four phases and that mature biofilm consisted of a highly organized architecture of both bacterial cells and a self-produced matrix of extracellular polysaccharides. Furthermore, sucrose was the most suitable carbon source for supporting the growth of biofilm cells and that 32 degrees C and pH 7.0 were the most favorable of the temperatures and pH levels examined. Meanwhile, the addition of Ca2+, Fe2+, K+ and Na+ enhanced the formation of biofilm in minimal medium cultures, whereas 2.5mM Cu2+ and Mn2+ was inhibitory. A better understanding of biofilm formation under different environmental parameters will improve our knowledge of the growth kinetics of D. zeae MS1 biofilm.

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