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Identification and Mapping of a New Soybean Male-Sterile Gene, mst-M

文献类型: 外文期刊

作者: Zhao, Qingsong 1 ; Tong, Ya 1 ; Yang, Chunyan 1 ; Yang, Yongqing 2 ; Zhang, Mengchen 1 ;

作者机构: 1.Hebei Acad Agr & Forestry Sci, Key Lab Crop Genet & Breeding Hebei, Inst Cereal & Oil Crops, Shijiazhuang, Hebei, Peoples R China

2.Fujian Agr & Forestry Univ, Root Biol Ctr, Coll Resources & Environm, Fuzhou, Fujian, Peoples R China

关键词: male sterility; abnormal pollen grains; inheritance test; bulked segregant analysis; dCAPS

期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.753; 五年影响因子:6.612 )

ISSN: 1664-462X

年卷期: 2019 年 10 卷

页码:

收录情况: SCI

摘要: The use of sterility is common in plants and multiple loci for hybrid sterility have been identified in crops such as rice. In soybean, fine-mapping and research on the molecular mechanism of male sterility is limited. Here, we identified a male-sterile soybean line, which produces larger, abnormal pollen grains that stain poorly with I-2-KI. In an inheritance test, all F-1 plants were fertile and the F-2 and F-2:3 populations conformed with the expected segregation ratio of 3:1 (fertility: sterility) (p = 0.82) and showed a 1:2:0 ratio of homozygous fertile: heterozygous fertile: homozygous sterile genotypes (p = 0.73), suggesting that the sterility was controlled by a single recessive gene (designated "mst-M"). Bulked segregant analysis showed that almost all single-nucleotide polymorphisms (SNPs; 95.92%) were distributed on chromosome 13 and 868 SNPs (95.81%) were distributed in the physical region of Chromosome 13.21877872 to Chromosome 13.22862641. Genetic mapping revealed that mst-M was flanked by W1 and dCAPS-1 with genetic distances of 0.6 and 1.8 cM, respectively. The order of the consensus markers and known sterility genes was: Satt146 - (5.0 cM) - st5 - (2.5 cM) - Satt030 - (15.3 cM) - ms6 - (5.0 cM) - Satt149 - (39.5 cM) - W1 (0.6 cM) - (mst-M) under bar - (14.1 cM) - Satt516 (7.5 cM) - ms1 - (16.3 cM) - Satt595. These results suggest that mst-M is a newly identified male-sterility gene, which represents an alternative genetic resource for developing a hybrid seed production system for soybean.

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