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Constitutive overexpression of cytochrome P450 monooxygenase genes contributes to chlorantraniliprole resistance in Chilo suppressalis (Walker)

文献类型： 外文期刊

作者： Xu, Lu 1 ; Zhao, Jun 2 ; Sun, Yang 3 ; Xu, Dejin 1 ; Xu, Guangchun 1 ; Xu, Xiaolong 1 ; Zhang, Yueliang 1 ; Huang, Shuiji 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Educ Minist, Nanjing, Jiangsu, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Plant Protect, Nanjing, Jiangsu, Peoples R China

关键词： Chilo suppressalis; chlorantraniliprole; resistance; cytochrome P450 monooxygenase; RNAi

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2019 年 75 卷 3 期

页码：

收录情况： SCI

摘要： BACKGROUND The rice striped stem borer (SSB), Chilo suppressalis (Walker), which is one of the most economically important phytophagous pests, has developed resistance to multiple insecticides. The resistance of SSB against chlorantraniliprole has been investigated in detail. However, the mechanism of its metabolic resistance has rarely been studied. RESULTS A field population from Wuhu City, China was used to establish chlorantraniliprole resistant and susceptible strains (WHR and WHS) by laboratory continuous selection. Enzyme activities data suggested the potential involvement of cytochrome P450 monooxygenase in WHR. CYP6CV5, CYP9A68, CYP321F3, and CYP324A12 were significantly overexpressed in WHR (from 4.48 to 44.88-fold). These four P450 genes were expressed in the late developmental stages of WHR; however, they were almost absent during the egg stage. In addition, their expressions were much more sensitive to chlorantraniliprole induction in WHR than in WHS. Injection of individual and mixture dsRNAs reduced the expression of the four target genes (55.2-73.2% and 43.2-50.2%, respectively) and caused significant larvae mortality (55.1-65.1% and 88.2%, respectively). CONCLUSION Multiple overexpressed P450 genes were potentially associated with chlorantraniliprole resistance, as confirmed by the RNA interference (RNAi) assay. Our findings suggested that metabolic resistance to chlorantraniliprole might be mediated by P450s. (c) 2018 Society of Chemical Industry

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