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Extraction, identification and structure-activity relationship of antioxidant peptides from sesame (Sesamum indicum L.) protein hydrolysate

文献类型： 外文期刊

作者： Lu, Xin 1 ; Zhang, Lixia 1 ; Sun, Qiang 1 ; Song, Guohui 1 ; Huang, Jinian 1 ;

作者机构： 1.Henan Acad Agr Sci, Res Ctr Agr & Sideline Prod Proc, 116 Pk Rd, Zhengzhou 450002, Henan, Peoples R China

关键词： Quantitative structure-activity relationship; Peptide match; Sesame protein; Enzymolysis; Purification; Antioxidant activity

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）

ISSN： 0963-9969

年卷期： 2019 年 116 卷

页码：

收录情况： SCI

摘要： To elucidate the sequence, origin and structure-activity relationship of antioxidant peptides from sesame protein, sesame protein was hydrolysed by a dual-enzyme system comprised alcalase and trypsin, then this hydrolysate was fractionated by ultrafiltration and preparative HPLC. Subsequently, peptides in the high antioxidant activity fraction were identified by nano liquid chromatography-electrospray ionization-tandem mass spectrometry (Nano-LC-ESI-MS/MS), finally the structure-activity relationship of antioxidant peptide with the strongest activity in the sesame peptides was illustrated by comparative molecular field analysis (CoMFA). The results showed that seven novel antioxidant peptides were discovered, their sequences were as follows, RDRHQKIG, TDRHQKLR, MNDRVNQGE, RENIDKPSRA, SYPTECRMR, GGVPRSGEQEQQ and AGEQGFEYVTFR. The SYPTECRMR was the hydrolysate of 2S albumin, the others derived from 11S globulin. The SYPTECRMR whose IC50 Values of DPPH and ABTS were 0.105 mg/mL and 0.004 mg/mL respectively exhibited the highest antioxidant activity among the seven sesame peptides. The active site of SYPTECRMR tended to locate on Cys6 and Met8. A positive correlation between Cys6, Met8, the bulky C-terminal amino acid residue (Arg9), the negative charged group around sulphur-containing amino acids and the antioxidant activity of SYPTECRMR was observed from the CoMFA model. The results presented herein suggested that sesame protein hydrolysates have potential applications in functional food due to their high antioxidant activity, the CoMFA model could provide insight into the structure-activity relationship of antioxidant peptide, which is useful to screen, identify and design the novel antioxidant peptide.

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