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Identification and comprehensive evaluation of a novel biocontrol agent Bacillus atrophaeus JZB120050

文献类型： 外文期刊

作者： Ni, Mi 1 ; Wu, Qiong 1 ; Wang, Junli 2 ; Liu, Wei C. 1 ; Ren, Jian H. 3 ; Zhang, Dian P. 1 ; Zhao, Juan 1 ; Liu, D. E. W. 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China

2.Guangdong Ocean Univ, Agr Coll, Zhanjiang, Peoples R China

3.Suzhou BioNovoGene Metabol Platform, Suzhou, Peoples R China

关键词： Bacillus atrophaeus; biocontrol; secondary metabolites; cell-wall-degrading enzymes; heterologous expression

期刊名称：JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES （ 影响因子：1.99； 五年影响因子：2.116 ）

ISSN： 0360-1234

年卷期： 2018 年 53 卷 12 期

页码：

收录情况： SCI

摘要： Bacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol.

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