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Genome-Wide Analysis, Expression Profile, and Characterization of the Acid Invertase Gene Family in Pepper

文献类型： 外文期刊

作者： Shen, Long-Bin 1 ; Qin, Yu-Ling 1 ; Qi, Zhi-Qiang 1 ; Niu, Yu 1 ; Liu, Zi-Ji 1 ; Liu, Wei-Xia 1 ; He, Huang 2 ; Cao, Zhen 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Crop Gene Resources & Germplasm Enhanceme, Minist Agr, Danzhou 571737, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Crop Gene Resources

关键词： acid invertase; pepper; gene expression; enzymatic activity

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Catalytic decomposition of sucrose by acid invertases (AINVs) under acidic conditions plays an important role in the development of sink organs in plants. To reveal the function of AINVs in the development of pepper fruits, nine AINV genes of pepper were identified. Protein sequencing and phylogenetic analysis revealed that the CaAINV family may be divided into cell wall invertases (CaCWINV1-7) and vacuolar invertases (CaVINV1-2). CaAINVs contain conserved regions and protein structures typical of the AINVs in other plants. Gene expression profiling indicated that CaCWINV2 and CaVINV1 were highly expressed in reproductive organs but differed in expression pattern. CaCWINV2 was mainly expressed in buds and flowers, while CaVINV1 was expressed in developmental stages, such as the post-breaker stage. Furthermore, invertase activity of CaCWINV2 and CaVINV1 was identified via functional complementation in an invertase-deficient yeast. Optimum pH for CaCWINV2 and CaVINV1 was found to be 4.0 and 4.5, respectively. Gene expression and enzymatic activity of CaCWINV2 and CaVINV1 indicate that these AINV enzymes may be pivotal for sucrose hydrolysis in the reproductive organs of pepper.

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