文献类型： 外文期刊
作者： Qiu You-wen 1 ; Feng Zhe 1 ; Fu Ming-ming 2 ; Yuan Xiao-han 1 ; Luo Chao-chao 1 ; Yu Yan-bo 1 ; Feng Yan-zhong 3 ; Wei Qi 3 ;
作者机构： 1.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China
2.Heilongjiang Inst Educ, Harbin 150030, Heilongjiang, Peoples R China
3.Heilongjiang Acad Agr Sci, Harbin 150086, Heilongjiang, Peoples R China
关键词： soybean; salinity tolerance; two-dimensional gel electrophoresis; GsMAPK4
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2019 年 18 卷 2 期
页码：
收录情况： SCI
摘要： Salt stress is one of the major factors affecting plant growth and yield in soybean under saline soil condition. Despite many studies on salinity tolerance of soybean during the past few decades, the detailed signaling pathways and the signaling molecules for salinity tolerance regulation have not been clarified. In this study, a proteomic technology based on two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins responsible for salinity tolerance in soybean plant. Real-time quantitative PCR (qRT-PCR) and Western blotting (WB) were used to verify the results of 2-DE/MS. Based on the results of 2-DE and MS, we selected glucosyltransferase (GsGT4), 4-coumarate, coenzyme A ligase (Gs4CL1), mitogen-activated protein kinase 4 (GsMAPK4), dehydration responsive element binding protein (GsDREB1), and soybean cold-regulated gene (GsSRC1) in the salinity tolerant soybean variety, and GsMAPK4 for subsequent research. We transformed soybean plants with mitogen-activated-protein kinase 4 (GsMAPK4) and screened the resulting transgenics soybean plants using PCR and WB, which confirmed the expression of GsMAPK4 in transgenic soybean. GsMAPK4-overexpressed transgenic plants showed significantly increased tolerance to salt stress, suggesting that GsMAPK4 played a pivotal role in salinity tolerance. Our research will provide new insights for better understanding the salinity tolerance regulation at molecular level.
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